Document Detail


Synergistic effect of indomethacin and NGX6 on proliferation and invasion by human colorectal cancer cells through modulation of the Wnt/beta-catenin signaling pathway.
MedLine Citation:
PMID:  19353243     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was designed to investigate whether indomethacin and NGX6 synergistically inhibit the growth and invasiveness of human colon cancer cells (HT-29 and SW620) and to elucidate the molecular mechanism of their action. Cell proliferation was assessed by MTT assay. Cell apoptosis was assessed by acridine orange/ethidium bromide staining (AO-EB) and annexin-V-FITC/PI assay. Invasive behaviors of colorectal cancer cells were examined by cell adhesion, migration, and invasion assays. Gap junctional intercellular communication (GJIC) was assessed by the scrape-loading/dye transfer technique. The subcellular localization and expression of beta-catenin protein was examined by immunofluorescence staining and western blot analysis, respectively. Indomethacin and NGX6 had a synergistic effect on inhibiting proliferation and invasiveness of colon cancer HT-29 and SW620 cells, restoring GJIC of HT-29 and SW620, and suppressing translocation of beta-catenin from the nucleus and cytoplasm to the plasma membrane. However, they did not have synergistic effects on enhancing apoptosis and suppressing extracellular matrix adhesion of HT-29 and SW620 cells. Indomethacin and NGX6 inhibit the proliferation and invasiveness of HT-29 and SW620 colon cancer cells by attenuating the WNT/ss-catenin signaling pathway.
Authors:
Qin Guo; Minghua Wu; Ping Lian; Mantian Liao; Zhiming Xiao; Xiaoyan Wang; Shourong Shen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-08
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  330     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-12-03     Completed Date:  2010-03-02     Revised Date:  2011-10-13    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  71-81     Citation Subset:  IM    
Affiliation:
Department of Digestion Medicine, The Third Affiliated Hospital of Xiang Ya School of Medicine, Central South University, Changsha, 410078, Hunan, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents, Non-Steroidal
Apoptosis / drug effects
Cell Adhesion / drug effects
Cell Communication / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects*
Colorectal Neoplasms / pathology,  therapy*
Drug Synergism
Gap Junctions / drug effects
Humans
Indomethacin / pharmacology*
Membrane Proteins / genetics,  pharmacology*
Neoplasm Invasiveness / prevention & control*
Signal Transduction / drug effects
Transfection
Tumor Suppressor Proteins / genetics,  pharmacology*
Wnt Proteins / metabolism
beta Catenin / metabolism
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Membrane Proteins; 0/TMEM8B protein, human; 0/Tumor Suppressor Proteins; 0/Wnt Proteins; 0/beta Catenin; 53-86-1/Indomethacin

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