| Synergistic effect of indomethacin and NGX6 on proliferation and invasion by human colorectal cancer cells through modulation of the Wnt/beta-catenin signaling pathway. | |
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MedLine Citation:
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PMID: 19353243 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was designed to investigate whether indomethacin and NGX6 synergistically inhibit the growth and invasiveness of human colon cancer cells (HT-29 and SW620) and to elucidate the molecular mechanism of their action. Cell proliferation was assessed by MTT assay. Cell apoptosis was assessed by acridine orange/ethidium bromide staining (AO-EB) and annexin-V-FITC/PI assay. Invasive behaviors of colorectal cancer cells were examined by cell adhesion, migration, and invasion assays. Gap junctional intercellular communication (GJIC) was assessed by the scrape-loading/dye transfer technique. The subcellular localization and expression of beta-catenin protein was examined by immunofluorescence staining and western blot analysis, respectively. Indomethacin and NGX6 had a synergistic effect on inhibiting proliferation and invasiveness of colon cancer HT-29 and SW620 cells, restoring GJIC of HT-29 and SW620, and suppressing translocation of beta-catenin from the nucleus and cytoplasm to the plasma membrane. However, they did not have synergistic effects on enhancing apoptosis and suppressing extracellular matrix adhesion of HT-29 and SW620 cells. Indomethacin and NGX6 inhibit the proliferation and invasiveness of HT-29 and SW620 colon cancer cells by attenuating the WNT/ss-catenin signaling pathway. |
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Authors:
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Qin Guo; Minghua Wu; Ping Lian; Mantian Liao; Zhiming Xiao; Xiaoyan Wang; Shourong Shen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-08 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 330 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-12-03 Completed Date: 2010-03-02 Revised Date: 2011-10-13 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 71-81 Citation Subset: IM |
Affiliation:
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Department of Digestion Medicine, The Third Affiliated Hospital of Xiang Ya School of Medicine, Central South University, Changsha, 410078, Hunan, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Inflammatory Agents, Non-Steroidal Apoptosis / drug effects Cell Adhesion / drug effects Cell Communication / drug effects Cell Line, Tumor Cell Proliferation / drug effects* Colorectal Neoplasms / pathology, therapy* Drug Synergism Gap Junctions / drug effects Humans Indomethacin / pharmacology* Membrane Proteins / genetics, pharmacology* Neoplasm Invasiveness / prevention & control* Signal Transduction / drug effects Transfection Tumor Suppressor Proteins / genetics, pharmacology* Wnt Proteins / metabolism beta Catenin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Membrane Proteins; 0/TMEM8B protein, human; 0/Tumor Suppressor Proteins; 0/Wnt Proteins; 0/beta Catenin; 53-86-1/Indomethacin |
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