| Synergistic cytotoxicity of acidity and glucose degradation products in peritoneal dialysis fluid. | |
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MedLine Citation:
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PMID: 15558338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Of the nonphysiological compounds in glucose-rich peritoneal dialysate, we investigated the cytotoxicity and synergistic cytotoxicity of acidity and glucose degradation products (GDPs) using human peritoneal mesothelial cells (HPMC). The effect of pH on cell viability was examined by adding 1N HCl to a phosphate-buffered solution (pH > or = 5.5). We also examined the cytotoxic effects of various GDPs [glyoxal (GO), methylglyoxal (MGO), and 3-deoxyglucosone (3DG), alone or in combination] and pH (5.5 or 6.7). The cells were exposed to these solutions for 2 or 4 h. Cell viability was determined by 3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay. Although the MTT viability of HPMC was not decreased by GDP or acidity alone, the combination of acidity and GDP markedly decreased MTT viability, strongly suggesting the synergistic cytotoxicity of GDP and acidity. |
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Authors:
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Eiji Okabe; Tadashi Tomo; Kazuhito Tezono; Hiroshi Kikuchi; Jyun-ichi Kadota; Masaru Nasu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs Volume: 7 ISSN: 1434-7229 ISO Abbreviation: J Artif Organs Publication Date: 2004 |
Date Detail:
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Created Date: 2004-11-23 Completed Date: 2005-03-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9815648 Medline TA: J Artif Organs Country: Japan |
Other Details:
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Languages: eng Pagination: 155-60 Citation Subset: IM |
Affiliation:
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Second Department of Internal Medicine, Department of Infectious Diseases, Faculty of Medicine, Oita University, 1-1 Hasama, Oita 879-5593, Japan. okabey@med.oita-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Survival Cells, Cultured Dialysis Solutions / chemistry Epithelial Cells* Hot Temperature Hydrogen-Ion Concentration Peritoneal Dialysis Peritoneum / cytology* Tetrazolium Salts Thiazoles |
| Chemical | |
Reg. No./Substance:
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0/Dialysis Solutions; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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