| Synergistic activity of histone deacetylase and proteasome inhibition against pancreatic and hepatocellular cancer cell lines. | |
| | |
MedLine Citation:
|
PMID: 21508352 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
MATERIALS AND METHODS: Antiproliferative effects were assessed using a sulforhodamine B assay. Synergy was evaluated using the Chou and Talalay method. Apoptosis was measured by caspase-3/-7 activity and PARP cleavage. Downstream effector proteins were detected via immunoblotting. Quantitative nuclear magnetic resonance (NMR)-based metabolomics analysis was performed. RESULTS: There were single-agent antiproliferative effects against PC and HCC cell lines; the combination of bel and bor (bel+bor) had a synergistic effect. There was up to a 45-fold induction of apoptosis over the control. Post-treatment cell death was associated with p21 up-regulation, more pronounced with treatment with bel+bor. Treatment with bel+bor enhanced hyperacetylation of histone H3 over single-agent bel. A metabolic signature was established for treatments with bor and bel+bor. CONCLUSION: The combination of bel+bor displayed significant antiproliferative activity against PC and HCC cell lines, with exhibiting synergistic antiproliferative and proapoptotic patterns even at suboptimal single-agent doses. |
| | |
Authors:
|
J L Spratlin; T M Pitts; G N Kulikowski; M P Morelli; J J Tentler; N J Serkova; S G Eckhardt |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Anticancer research Volume: 31 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-04-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
|
Languages: eng Pagination: 1093-103 Citation Subset: IM |
Affiliation:
|
MD FRCPC, Medical Oncology, Cross Cancer Institute, University of Alberta, 11560 University Avenue, Edmonton, Alberta, Canada, T6G. jennifer.spratlin@albertahealthservices.ca. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reproductive experience alters prolactin receptor expression in mammary and hepatic tissues in femal...
Next Document: Targeting notch to eradicate pancreatic cancer stem cells for cancer therapy.