| Synergistic effects of sonoporation and taurolidin/TRAIL on apoptosis in human fibrosarcoma. | |
| | |
MedLine Citation:
|
PMID: 20870344 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Sonodynamic therapy, in combination with ultrasound contrast agents, proved to enhance the uptake of chemotherapeutics in malignant cells. HT1080 fibrosarcoma cells were treated in vitro with a combination of ultrasound SonoVue™-microbubbles and taurolidine (TRD) plus tumor necrosis factor related apoptosis inducing ligand (TRAIL). Apoptosis was measured by TdT-mediated dUTP-biotin nick end labelling (TUNEL) assay and fluorescence activated cell sorting (FACS) analysis. Gene expression was analysed by RNA-microarray. The apoptotic effects of TRD and TRAIL on human fibrosarcoma are enhanced by sonodynamic therapy and additional application of contrast agents, such as SonoVue™ by 25%. A broad change in the expression of genes related to apoptotic pathways is observed when ultrasound and microbubbles act synchronously in combination with the chemotherapeutics (e.g. BIRC3, NFKBIA and TNFAIP3). Some of these genes have already been proven to play a role in programmed cell death in human fibrosarcoma (HSPA1A/HSPA1B, APAF1, PAWR, SOCS2) or were associated with sonication induced apoptosis (CD44). Further studies are needed to explore the options of sonodynamic therapy on soft tissue sarcoma and its molecular mechanisms. |
| | |
Authors:
|
Adrien Daigeler; Ansgar M Chromik; Kathrin Haendschke; Sabine Emmelmann; Monica Siepmann; Karin Hensel; Georg Schmitz; Ludger Klein-Hitpass; Hans U Steinau; Marcus Lehnhardt; Joerg Hauser |
Related Documents
:
|
16520654 - Proteasome inhibition sensitizes hepatocellular carcinoma cells to trail by suppressing... 11562074 - Fas ligand and trail augment the effect of photodynamic therapy on the induction of apo... 19309554 - Selection and characterization of a novel agonistic human recombinant anti-trail-r2 min... 20045024 - Identification and functional characterization of a human strail homolog, castrail, in ... 21252304 - Gastrin-releasing peptide blockade as a broad-spectrum anti-inflammatory therapy for as... 22074294 - Thymosin ?(4): a multi-functional regenerative peptide. basic properties and clinical a... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-27 |
Journal Detail:
|
Title: Ultrasound in medicine & biology Volume: 36 ISSN: 1879-291X ISO Abbreviation: Ultrasound Med Biol Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-10-25 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0410553 Medline TA: Ultrasound Med Biol Country: England |
Other Details:
|
Languages: eng Pagination: 1893-906 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Hand, Plastic and Reconstructive Surgery, Burn Center, BG-Unfallkrankenhaus, Ludwigshafen, Germany. adaigeler@bgu-ludwigshafen.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Measurement of embryo volume using SonoAVC.
Next Document: Controlled attenuation parameter (CAP): a novel VCTE™ guided ultrasonic attenuation measurement fo...