| Synergistic effect of tumor necrosis factor-alpha and hydrogen peroxide on the induction of IL-8 production in human intestinal caco-2 cells. | |
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MedLine Citation:
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PMID: 20845069 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Oxidative stress and inflammatory cytokines such as TNF-α are thought to be involved in mucosal inflammation. The intestinal epithelium may be concurrently stimulated by both oxidative stress and inflammatory cytokines during the inflammation process in the intestines. However, experimental models for intestinal inflammation still employ single stimulation, either by an oxidative stress or inflammatory cytokine. We therefore examined an in vitro inflammation study using human intestinal epithelial Caco-2 cells, in which the cells were stimulated both by hydrogen peroxide and TNF-α, and measured the IL-8 production as an index of inflammation. The IL-8 production (secretion, mRNA expression, and transcriptional activity) induced by both TNF-α and H(2)O(2) was significantly higher than that by single stimulation. The synergistic effect of TNF-α and H(2)O(2) on the NF-κB signaling pathway (transcriptional activity and p65 nuclear translocation) was also observed. Oxidative stress and TNF-α may cooperatively enhance IL-8 production via NF-κB in Caco-2 cells. |
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Authors:
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Hee Soon Shin; Zhaohui Zhao; Hideo Satsu; Mamoru Totsuka; Makoto Shimizu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Inflammation Volume: 34 ISSN: 1573-2576 ISO Abbreviation: Inflammation Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600105 Medline TA: Inflammation Country: United States |
Other Details:
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Languages: eng Pagination: 440-7 Citation Subset: IM |
Affiliation:
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Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan, aa087028@mail.ecc.u-tokyo.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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