Document Detail

The synergistic apoptotic interaction of panaxadiol and epigallocatechin gallate in human colorectal cancer cells.
MedLine Citation:
PMID:  22566066     Owner:  NLM     Status:  MEDLINE    
Panaxadiol (PD) is a purified sapogenin of ginseng saponins, which exhibits anticancer activity. Epigallocatechin gallate (EGCG), a major catechin in green tea, is a strong botanical antioxidant. In this study, we investigated the possible synergistic anticancer effects of PD and EGCG on human colorectal cancer cells and explored the potential role of apoptosis in the synergistic activities. Effects of selected compounds on HCT-116 and SW-480 human colorectal cancer cells were evaluated by a modified trichrome stain cell proliferation analysis. Cell cycle distribution and apoptotic effects were analyzed by flow cytometry after staining with PI/RNase or annexin V/PI. Cell growth was suppressed after treatment with PD (10 and 20 µm) for 48 h. When PD (10 and 20 µm) was combined with EGCG (10, 20, and 30 µm), significantly enhanced antiproliferative effects were observed in both cell lines. Combining 20 µm of PD with 20 and 30 µm of EGCG significantly decreased S-phase fractions of cells. In the apoptotic assay, the combination of PD and EGCG significantly increased the percentage of apoptotic cells compared with PD alone (p < 0.01). The synergistic apoptotic effects were also supported by docking analysis, which demonstrated that PD and EGCG bound in two different sites of the annexin V protein. Data from this study suggested that apoptosis might play an important role in the EGCG-enhanced antiproliferative effects of PD on human colorectal cancer cells.
Guang-Jian Du; Chong-Zhi Wang; Lian-Wen Qi; Zhi-Yu Zhang; Tyler Calway; Tong-Chuan He; Wei Du; Chun-Su Yuan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-05-08
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  27     ISSN:  1099-1573     ISO Abbreviation:  Phytother Res     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-04     Completed Date:  2013-05-13     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  272-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
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MeSH Terms
Annexin A5 / chemistry
Apoptosis / drug effects*
Catechin / analogs & derivatives*,  chemistry,  pharmacology
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Colorectal Neoplasms / pathology
Drug Synergism*
Ginsenosides / chemistry,  pharmacology*
Molecular Docking Simulation
Grant Support
Reg. No./Substance:
0/Annexin A5; 0/Ginsenosides; 19666-76-3/panaxadiol; 8R1V1STN48/Catechin; BQM438CTEL/epigallocatechin gallate

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