Document Detail

Synergism between a siRNA targeted to survivin and 5-FU in inhibiting MCF-7 cell proliferation in vitro.
MedLine Citation:
PMID:  16546719     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the role of small interfering RNA (siRNA) targeted to survivin in combination with 5-fluorouracil (5-FU) in inhibiting the proliferation of MCF-7 cells. METHODS: A siRNA targeted to survivin was synthesized and transfected into MCF-7 cells via lipofectin. Changes of the cell growth activity in response to combined treatment with survivin siRNA and 5-FU or 5-FU treatment alone was evaluated by MTT assay. The Q method of Jin Zhenjun was used to evaluated synergism between the synthesized siRNA and 5-FU. RESULTS: Treatment with 5 nmol/L siRNA reduced the IC50 of 5-FU from 4.42 to 1.18 microg/ml, and the inhibitory effect of combined treatment on MCF-7 cells was higher than that of 5-FU alone (F=26.74, P<0.01). Synergism effect was observed between 5-FU at lower concentrations and survivin siRNA. CONCLUSION: siRNA may enhance the effectiveness of 5-FU in inhibiting the proliferation of MCF-7 cells.
Shu-qun Zhang; Shu-hui Zhang; Xin-huan Xue; Xi-jing Wang; Jian-tao Jiang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nan fang yi ke da xue xue bao = Journal of Southern Medical University     Volume:  26     ISSN:  1673-4254     ISO Abbreviation:  Nan Fang Yi Ke Da Xue Xue Bao     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-20     Completed Date:  2008-04-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101266132     Medline TA:  Nan Fang Yi Ke Da Xue Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  251-4     Citation Subset:  IM    
Department of Oncology, Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
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MeSH Terms
Antimetabolites, Antineoplastic / pharmacology
Apoptosis / drug effects,  genetics
Cell Line, Tumor
Cell Proliferation / drug effects*
Cell Survival / drug effects,  genetics
Dose-Response Relationship, Drug
Fluorouracil / pharmacology*
Genetic Vectors / genetics
Microtubule-Associated Proteins / genetics*
Neoplasm Proteins / genetics*
RNA Interference
RNA, Small Interfering / genetics*
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/BIRC5 protein, human; 0/Microtubule-Associated Proteins; 0/Neoplasm Proteins; 0/RNA, Small Interfering; 51-21-8/Fluorouracil

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