| Synergism between Pax-8 and lim-1 in embryonic kidney development. | |
| | |
MedLine Citation:
|
PMID: 10491256 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Pax genes encode a family of highly conserved DNA-binding transcription factors. These proteins play key roles in regulating a number of vertebrate and invertebrate developmental processes. Mutations in Pax-6 result in eye defects in flies, mice, and humans, and ectopic expression of this gene can trigger the development of ectopic compound eyes in flies. Likewise, mutation of other Pax genes in vertebrates results in the failure of specific differentiation programs-Pax-1 causes skeletal defects; Pax-2, kidney defects; Pax-3 or Pax-7, neural crest defects; Pax-4, pancreatic beta-cell defects; Pax-5, B-cell defects; Pax-8, thyroid defects; and Pax-9, tooth defects. Although this class of genes is obviously required for the normal differentiation of a number of distinct organ systems, they have not previously been demonstrated to be capable of directing the embryonic development of organs in vertebrates. In this report, it is demonstrated that Pax-8 plays such a role in the establishment of the Xenopus embryonic kidney, the pronephros. However, in order to efficiently direct cells to form pronephric kidneys, XPax-8 requires cofactors, one of which may be the homeobox transcription factor Xlim-1. These two genes are initially expressed in overlapping domains in late gastrulae, and cells expressing both genes will go on to form the kidney. Ectopic expression of either gene alone has a moderate effect on pronephric patterning, while coexpression of XPax-8 plus Xlim-1 results in the development of embryonic kidneys of up to five times normal complexity and also leads to the development of ectopic pronephric tubules. This effect was synergistic rather than additive. XPax-2 can also synergize with Xlim-1, but the expression profile of this gene indicates that it normally functions later in pronephric development than does XPax-8. Together these data indicate that the interaction between XPax-8 and Xlim-1 is a key early step in the establishment of the pronephric primordium. |
| | |
Authors:
|
T J Carroll; P D Vize |
Related Documents
:
|
10218586 - Eos: a novel member of the ikaros gene family expressed predominantly in the developing... 20610746 - Nfia controls telencephalic progenitor cell differentiation through repression of the n... 12441636 - Genetic basis of neural tube defects. ii. genes correlated with folate and methionine m... 12947416 - Loss of eyes in zebrafish caused by mutation of chokh/rx3. 11829516 - The nasal epithelium as a factory for systemic protein delivery. 9204566 - Functional and regulatory analysis of the two copies of the fixnoqp operon of rhizobium... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Developmental biology Volume: 214 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 1999 Oct |
Date Detail:
|
Created Date: 1999-11-02 Completed Date: 1999-11-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 46-59 Citation Subset: IM |
Copyright Information:
|
Copyright 1999 Academic Press. |
Affiliation:
|
Cell and Developmental Biology, University of Texas, Austin, Texas, 78712, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Body Patterning Conserved Sequence DNA-Binding Proteins / chemistry, genetics*, metabolism* Embryo, Nonmammalian / physiology Gene Expression Regulation, Developmental* Homeodomain Proteins / chemistry, genetics*, metabolism* Humans Kidney / embryology* Mesoderm / physiology* Mice Molecular Sequence Data Nuclear Proteins* Paired Box Transcription Factors Sequence Alignment Sequence Homology, Amino Acid Trans-Activators / chemistry, genetics*, metabolism* Xenopus Xenopus Proteins |
| Chemical | |
Reg. No./Substance:
|
0/DNA-Binding Proteins; 0/Homeodomain Proteins; 0/LHX1 protein, human; 0/Lhx1 protein, mouse; 0/Lim-1 protein, Xenopus; 0/Nuclear Proteins; 0/PAX8 protein, human; 0/Paired Box Transcription Factors; 0/Pax8 protein, Xenopus; 0/Pax8 protein, mouse; 0/Trans-Activators; 0/Xenopus Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A retinoic acid-inducible modular protease in budding ascidians.
Next Document: Patterns of programmed cell death in populations of developing spinal motoneurons in chicken, mouse,...