Document Detail

Syndecan-2 expression increases serum-withdrawal-induced apoptosis, mediated by re-distribution of Fas into lipid rafts, in stably transfected Swiss 3T3 cells.
MedLine Citation:
PMID:  17041758     Owner:  NLM     Status:  MEDLINE    
To examine the function of syndecan-2, one of the most abundant heparan sulfate proteoglycans in fibroblasts, we obtained stably transfected Swiss 3T3 clones. We examined the effects of stable syndecan-2 overexpression on programmed cell death, finding that syndecan-2 transfected cells were more sensitive to apoptosis induced by serum-withdrawal than control cells. In addition, overexpression of syndecan-2 correlates with increased membrane levels of the Fas/CD95 receptor, suggesting that the increased serum-withdrawal apoptosis observed in Swiss 3T3 cells might be Fas receptor-dependent. Differences in Fas membrane levels between both control and syndecan-2 transfected cells result from a redistribution of the Fas receptor. Our data clearly demonstrate that increased Fas levels are primarily related to lipid rafts and that this increase is a key factor in Fas/CD95-mediated apoptosis. Moreover, disruption of lipid rafts with methyl-beta-cyclodextrin or filipin significantly reduced apoptosis in response to serum withdrawal. The differences in Fas/CD95 membrane distribution could explain why syndecan-2 transfected cells have a higher susceptibility to serum-withdrawal-induced apoptosis.
Joan Villena; Jessica Mainez; Oriol Noguer; Héctor Contreras; Francesc Granés; Manuel Reina; Isabel Fabregat; Senén Vilaró
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  11     ISSN:  1360-8185     ISO Abbreviation:  Apoptosis     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-06     Completed Date:  2007-09-19     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2065-75     Citation Subset:  IM    
Department of Cellular Biology, Faculty of Biology, University of Barcelona, Diagonal 645, 08028, Barcelona, Spain.
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MeSH Terms
3T3 Cells
Apoptosis* / drug effects
Cell Culture Techniques
DNA, Complementary
Fas Ligand Protein / metabolism*
Filipin / pharmacology
Membrane Microdomains / metabolism*
Serum / metabolism*
Syndecan-2 / genetics,  physiology*
beta-Cyclodextrins / pharmacology
Reg. No./Substance:
0/DNA, Complementary; 0/Fas Ligand Protein; 0/beta-Cyclodextrins; 0/methyl-beta-cyclodextrin; 149769-25-5/Syndecan-2; 480-49-9/Filipin

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