Document Detail

Syncytin-A mediates the formation of syncytiotrophoblast involved in mouse placental development.
MedLine Citation:
PMID:  17762178     Owner:  NLM     Status:  MEDLINE    
Syncytin-A, a new mouse endogenous retroviral envelope protein expressed in placenta, can mediate cell fusion in vitro. But its physiological function was still unknown. We proposed a role for syncytin-A in syncytiotrophoblast (SynT) formation derived from the differentiation of trophoblast stem (TS) cells during placental development. To evaluate this hypothesis, we analyzed the involvement of syncytin-A in the differentiation of mouse TS cells. After withdrawing fibroblast growth factor 4 (FGF4), TS cells can fuse to form SynT cells. We found syncytin-A mRNA and protein expression are colinear with fusion index increase during TS cell differentiation. Expression of syncytin-A is localized in SynT cells through in situ immunofluorescent staining. By using specific antibody and antisense oligonucleotides, we demonstrated that inhibition of syncytin-A lead to obvious decrease of SynT cell formation. These results present evidence in support of the direct role for syncytin-A in mouse TS cell fusion and differentiation involved in placental development.
Rui Gong; Liqin Huang; Jian Shi; Kan Luo; Gang Qiu; Huixing Feng; Po Tien; Gengfu Xiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  20     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2007  
Date Detail:
Created Date:  2007-08-31     Completed Date:  2007-09-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  517-26     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2007 S. Karger AG, Basel.
State Key Laboratory of Virology and Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan, China.
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MeSH Terms
Cell Differentiation* / drug effects
Cells, Cultured
Fibroblast Growth Factor 4 / pharmacology
Gene Expression Regulation
Microscopy, Fluorescence
Oligonucleotides, Antisense / genetics
Placenta / cytology*,  drug effects,  embryology,  metabolism*
Pregnancy Proteins / genetics,  metabolism*
RNA, Messenger / genetics
Trophoblasts / cytology*,  drug effects,  metabolism*
Reg. No./Substance:
0/Fibroblast Growth Factor 4; 0/Oligonucleotides, Antisense; 0/Pregnancy Proteins; 0/RNA, Messenger; 0/syncytin-A protein, mouse

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