| Syncytin-A mediates the formation of syncytiotrophoblast involved in mouse placental development. | |
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MedLine Citation:
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PMID: 17762178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Syncytin-A, a new mouse endogenous retroviral envelope protein expressed in placenta, can mediate cell fusion in vitro. But its physiological function was still unknown. We proposed a role for syncytin-A in syncytiotrophoblast (SynT) formation derived from the differentiation of trophoblast stem (TS) cells during placental development. To evaluate this hypothesis, we analyzed the involvement of syncytin-A in the differentiation of mouse TS cells. After withdrawing fibroblast growth factor 4 (FGF4), TS cells can fuse to form SynT cells. We found syncytin-A mRNA and protein expression are colinear with fusion index increase during TS cell differentiation. Expression of syncytin-A is localized in SynT cells through in situ immunofluorescent staining. By using specific antibody and antisense oligonucleotides, we demonstrated that inhibition of syncytin-A lead to obvious decrease of SynT cell formation. These results present evidence in support of the direct role for syncytin-A in mouse TS cell fusion and differentiation involved in placental development. |
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Authors:
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Rui Gong; Liqin Huang; Jian Shi; Kan Luo; Gang Qiu; Huixing Feng; Po Tien; Gengfu Xiao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 20 ISSN: 1015-8987 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-08-31 Completed Date: 2007-09-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: Switzerland |
Other Details:
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Languages: eng Pagination: 517-26 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2007 S. Karger AG, Basel. |
Affiliation:
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State Key Laboratory of Virology and Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation* / drug effects Cells, Cultured Female Fibroblast Growth Factor 4 / pharmacology Fibroblasts Gene Expression Regulation Humans Mice Microscopy, Fluorescence Oligonucleotides, Antisense / genetics Placenta / cytology*, drug effects, embryology, metabolism* Pregnancy Pregnancy Proteins / genetics, metabolism* RNA, Messenger / genetics Trophoblasts / cytology*, drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fibroblast Growth Factor 4; 0/Oligonucleotides, Antisense; 0/Pregnancy Proteins; 0/RNA, Messenger; 0/syncytin-A protein, mouse |
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