Document Detail


Syncytin-A knockout mice demonstrate the critical role in placentation of a fusogenic, endogenous retrovirus-derived, envelope gene.
MedLine Citation:
PMID:  19564597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In most mammalian species, a key process of placenta development is the fusion of trophoblast cells into a highly specialized, multinucleated syncytiotrophoblast layer, through which most of the maternofetal exchanges take place. Little is known about this process, despite the recent identification of 2 pairs of envelope genes of retroviral origin, independently acquired by the human (syncytin-1 and syncytin-2) and mouse (syncytin-A and syncytin-B) genomes, specifically expressed in the placenta, and with in vitro cell-cell fusion activity. By generating knockout mice, we show here that homozygous syncytin-A null mouse embryos die in utero between 11.5 and 13.5 days of gestation. Refined cellular and subcellular analyses of the syncytin-A-deficient placentae disclose specific disruption of the architecture of the syncytiotrophoblast-containing labyrinth, with the trophoblast cells failing to fuse into an interhemal syncytial layer. Lack of syncytin-A-mediated trophoblast cell fusion is associated with cell overexpansion at the expense of fetal blood vessel spaces and with apoptosis, adding to the observed maternofetal interface structural defects to provoke decreased vascularization, inhibition of placental transport, and fetal growth retardation, ultimately resulting in death of the embryo. These results demonstrate that syncytin-A is essential for trophoblast cell differentiation and syncytiotrophoblast morphogenesis during placenta development, and they provide evidence that genes captured from ancestral retroviruses have been pivotal in the acquisition of new, important functions in mammalian evolution.
Authors:
Anne Dupressoir; Cécile Vernochet; Olivia Bawa; Francis Harper; Gérard Pierron; Paule Opolon; Thierry Heidmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-29
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  106     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-22     Completed Date:  2009-08-25     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12127-32     Citation Subset:  IM    
Affiliation:
Unité des Rétrovirus Endogènes et Eléments Rétroïdes des Eucaryotes Supérieurs, Unité Mixte de Recherche 8122, Centre National de Recherche Scientifique, Institut Gustave Roussy, 94805 Villejuif, and Université Paris-Sud, 91405 Orsay, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Crosses, Genetic
Embryo Loss / metabolism,  pathology
Embryo, Mammalian / abnormalities,  pathology
Endogenous Retroviruses / genetics*
Extraembryonic Membranes / abnormalities,  pathology
Female
Gene Targeting
Genotype
Male
Mice
Mice, Knockout
Placenta / abnormalities,  pathology,  ultrastructure
Placentation / physiology*
Pregnancy
Pregnancy Proteins / deficiency*,  metabolism
Trophoblasts / pathology
Viral Envelope Proteins / genetics*
Chemical
Reg. No./Substance:
0/Pregnancy Proteins; 0/Viral Envelope Proteins; 0/syncytin-A protein, mouse
Comments/Corrections
Comment In:
Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11827-8   [PMID:  19617545 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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