Document Detail

Syncollin is differentially expressed in rat proximal small intestine and regulated by feeding behavior.
MedLine Citation:
PMID:  10666056     Owner:  NLM     Status:  MEDLINE    
Gradients of gene expression are maintained along the proximal-distal axis of the mammalian small intestine despite a continuously regenerating epithelium. To study the molecular mechanisms responsible for this phenomenon, we utilized a subtractive hybridization strategy to isolate genes differentially expressed in the duodenum but not ileum. We isolated and sequenced 15 clones. The clones were fragments of genes encoding lipases, proteases, and an esterase. A novel clone was characterized and subsequently shown to encode syncollin, a secretory granule protein that binds to syntaxin in a calcium-sensitive manner. RT-PCR and S1 nuclease protection assay were used to clarify the 5'-end of syncollin. Syncollin was expressed in the rat pancreas, spleen, duodenum, and colon. In situ hybridization localized syncollin expression in the pancreas to acinar cells and in the duodenum to villus epithelial cells.
S Tan; S C Hooi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  278     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-02     Completed Date:  2000-03-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G308-20     Citation Subset:  IM    
Department of Physiology, Faculty of Medicine, National University of Singapore, Singapore 119260.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Carrier Proteins / chemistry,  genetics*
DNA, Complementary / chemistry
Duodenum / metabolism
Gene Expression Regulation*
Ileum / metabolism
In Situ Hybridization
Intestine, Small / growth & development,  metabolism*
Membrane Proteins / chemistry,  genetics*
Molecular Sequence Data
RNA, Messenger / analysis
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Single-Strand Specific DNA and RNA Endonucleases
Reg. No./Substance:
0/Carrier Proteins; 0/DNA, Complementary; 0/Membrane Proteins; 0/RNA, Messenger; 0/Sycn protein, rat; EC Specific DNA and RNA Endonucleases

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