Document Detail


Synchronization of the human promyelocytic cell line HL 60 by thymidine.
MedLine Citation:
PMID:  3459588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultures of the promyelocytic cell line HL 60 were synchronized with thymidine. A concentration of 0.05 mM thymidine and an exposure time of 24 hr was found optimal for blocking about 90% of the cells in S phase. Following release from the thymidine block the cell cultures were followed intermittently over 40 hr for fluctuation in cell numbers, labelling with radioactive thymidine and nuclear DNA distributions. Mathematical evaluation of the results revealed a cycling time of 18.6 hr and a duration of specific cell phases of 8.6 hr, 7.1 hr and 2.9 hr for G1, S and G2 + M, respectively. The doubling time was 26 hr and the growth fraction was estimated as 1.
Authors:
B T Mortensen; N R Hartmann; I J Christensen; J K Larsen; T Kristensen; S B Wieslander; N I Nissen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  19     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-08-21     Completed Date:  1986-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  351-64     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Autoradiography
Cell Cycle / drug effects*
Cell Line
Cell Transformation, Neoplastic / drug effects*,  metabolism,  pathology
DNA / metabolism
Humans
Interphase / drug effects
Leukemia, Myeloid / metabolism,  pathology*
Scintillation Counting
Thymidine / metabolism,  pharmacology*
Chemical
Reg. No./Substance:
50-89-5/Thymidine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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