Document Detail

Synchronization of cells.
MedLine Citation:
PMID:  17623932     Owner:  NLM     Status:  MEDLINE    
Synchronization of cells is essential to study cell cycle specific events. If, for example, one suspects that a given DNA repair pathway is used in a particular cell cycle phase, the protocol can be used to enrich cells in each phase of the cell cycle and analyze the cellular response to DNA damage. Synchronization is also useful, when a gene is essential for a particular phase of the cell cycle. If a gene is, for example, essential for mitosis, synchronization of the cells in G1 phase with concomitant inactivation of the gene enables us to study the function of the gene in interphase, and to follow synchronous cell cycle progression to M phase. Two synchronization methods: centrifugal elutriation to enrich G1, S or G2 phase cells and nocodazole-mimocine sequential treatment to enrich cells at the G1/S boundary are described. Centrifugal elutriation can be achieved in less time (0.5-2 h) and with very little physiological stress to the cells whereas synchronization by drugs, such as nocodazole and mimocine, may result in unfavorable side effects.
Eiichiro Sonoda
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Sub-cellular biochemistry     Volume:  40     ISSN:  0306-0225     ISO Abbreviation:  Subcell. Biochem.     Publication Date:  2006  
Date Detail:
Created Date:  2007-07-12     Completed Date:  2007-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0316571     Medline TA:  Subcell Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  415-8     Citation Subset:  IM    
Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Kyoto 606-8501, Japan.
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MeSH Terms
Cell Cycle* / drug effects
Cell Line
Isoquinolines / pharmacology
Nocodazole / pharmacology
Reg. No./Substance:
0/Isoquinolines; 31430-18-9/Nocodazole; 76177-28-1/mimocine

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