Document Detail


Synchronization of Plodia interpunctella lepidopteran cells and effects of 20-hydroxyecdysone.
MedLine Citation:
PMID:  15056365     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated the molecular and cellular mechanisms involved in the control of insect cell cycle by 20-hydroxyecdysone (20E) using the IAL-PID2 cell line established from imaginal wing discs of Plodia interpunctella. We first defined conditions for use of hydroxyurea, a reversible inhibitor of DNA synthesis, in order to synchronize the IAL-PID2 cells in their division cycle. A high degree of synchrony was reached when cells were exposed to two consecutive hydroxyurea treatments at 1 mm for 36 h spaced 16 h apart. Under these conditions, flow cytometry analysis demonstrated that 20E at 10(-6) m induced an inhibition of cell growth by an arrest of 90% of the cells in G2/M phase. Using cDNA probes specifically designed from E75 and HR3 nuclear receptors of Plodia interpunctella, we showed that PiE75 and PHR3 were highly induced by 20E through S and G2 phases with maximal enhancement just before the G2/M arrest of cells. These findings suggest that PiE75 and PHR3 could be involved in a 20E-induced genetic cascade leading to G2/M arrest.
Authors:
D Siaussat; V Mottier; F Bozzolan; P Porcheron; S Debernard
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Insect molecular biology     Volume:  13     ISSN:  0962-1075     ISO Abbreviation:  Insect Mol. Biol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-01     Completed Date:  2004-06-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9303579     Medline TA:  Insect Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  179-87     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie Cellulaire des Invertébrés, Université Pierre et Marie Curie, Paris, France. David.Siaussat@snv.jussieu.fr
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Butterflies / physiology*
Cell Cycle / drug effects*,  physiology
Cell Line
DNA-Binding Proteins / genetics*,  isolation & purification
Ecdysterone / pharmacology*
Flow Cytometry
Gene Expression Regulation, Developmental*
Hydroxyurea / pharmacology
Insect Proteins / genetics*,  isolation & purification
Molecular Sequence Data
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Steroid / genetics*,  isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/E75 protein, insect; 0/Insect Proteins; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, Steroid; 127-07-1/Hydroxyurea; 5289-74-7/Ecdysterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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