| Synchronisation of Giardia lamblia: identification of cell cycle stage-specific genes and a differentiation restriction point. | |
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MedLine Citation:
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PMID: 18289546 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The intestinal parasite Giardia lamblia undergoes cell differentiations that entail entry into and departure from the replicative cell cycle. The pathophysiology of giardiasis depends directly upon the ability of the trophozoite form to replicate in the host upper small intestine. Thus, cell proliferation is tightly linked to disease. However, studies of cell cycle regulation in Giardia have been hampered by the inability to synchronise cultures. Here we report that Giardia isolates of the major human genotypes A and B can be synchronised using aphidicolin, a mycotoxin that reversibly inhibits replicative DNA polymerases in eukaryotic cells. Aphidicolin arrests Giardia trophozoites in the early DNA synthesis (S) phase of the cell cycle. We identified a set of cell cycle orthologues in the Giardia genome using bioinformatic analyses and showed that synchronised parasites express these genes in a cell cycle stage-specific manner. The synchronisation method also showed that during encystation, exit from the ordinary cell cycle occurs preferentially in G(2) and defines a restriction point for differentiation. Synchronisation opens up possibilities for further molecular and cell biological studies of chromosome replication, mitosis and segregation of the complex cytoskeleton in Giardia. |
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Authors:
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David S Reiner; Johan Ankarklev; Karin Troell; Daniel Palm; Rolf Bernander; Frances D Gillin; Jan O Andersson; Staffan G Svärd |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-01-18 |
Journal Detail:
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Title: International journal for parasitology Volume: 38 ISSN: 0020-7519 ISO Abbreviation: Int. J. Parasitol. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-05-26 Completed Date: 2008-09-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0314024 Medline TA: Int J Parasitol Country: England |
Other Details:
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Languages: eng Pagination: 935-44 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of California at San Diego, San Diego, CA 92103-8416, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aphidicolin / pharmacology Cell Cycle / drug effects* Cell Differentiation* Flow Cytometry Gene Expression / physiology Genes, cdc / physiology* Genotype Giardia lamblia / growth & development* Humans Intestine, Small / parasitology* |
| Grant Support | |
ID/Acronym/Agency:
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AI051687/AI/NIAID NIH HHS; AI42488/AI/NIAID NIH HHS; DK35108/DK/NIDDK NIH HHS; GM61896-04/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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38966-21-1/Aphidicolin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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