| Synbiotic promotion of epithelial proliferation by orally ingested encapsulated Bifidobacterium breve and raffinose in the small intestine of rats. | |
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MedLine Citation:
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PMID: 18837471 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We evaluated the effects of Bifidobacterium breve JCM1192(T )and/or raffinose on epithelial proliferation in the rat small and large intestines. WKAH/Hkm Slc rats (4 wk old) were fed a control diet, a diet supplemented with either encapsulated B. breve (30 g/kg diet, 1.5 x 10(7) colony-forming unit/g capsule) or raffinose (30 g/kg diet), or a diet supplemented with both encapsulated B. breve and raffinose, for 3 wk. Epithelial proliferation in the small intestine, as assessed by bromodeoxyuridine immunohistochemistry, was increased only in the B. breve plus raffinose-fed group. We determined the number of bifidobacteria in cecal contents using fluorescence in situ hybridization and confirmed the presence of ingested B. breve only in the B. breve plus raffinose-fed group. This suggests that the ingested B. breve cells used raffinose and were activated in the small intestine, where they subsequently influenced epithelial proliferation. In conclusion, we found a prominent synbiotic effect of encapsulated B. breve in combination with raffinose on epithelial proliferation in rat small intestine but not in large intestine. To our knowledge, this is the first report of a synbiotic that affects epithelial proliferation. |
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Authors:
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Satoshi Ishizuka; Ami Iwama; Achmad Dinoto; Akarat Suksomcheep; Kohshi Maeta; Takanori Kasai; Hiroshi Hara; Atsushi Yokota |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 53 Suppl 1 ISSN: 1613-4133 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-06-03 Completed Date: 2009-08-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: S62-7 Citation Subset: IM |
Affiliation:
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Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan. zuka@chem.agr.hokudai.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bifidobacterium / physiology* Bromodeoxyuridine / analysis Cecum / chemistry, cytology, microbiology Cell Division / drug effects* Colon / cytology Epithelial Cells / cytology* Hydrogen-Ion Concentration Intestine, Small / cytology* Male Probiotics / administration & dosage* Raffinose / administration & dosage* Rats |
| Chemical | |
Reg. No./Substance:
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512-69-6/Raffinose; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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