Document Detail


Synaptosomal uptake of choline and of gamma-aminobutyric acid: effects of ethanol and of dimethylsulfoxide.
MedLine Citation:
PMID:  2626218     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have characterized the interactive effects of ethanol and dimethylsulfoxide on synaptosomal uptakes of gamma-aminobutyric acid (GABA) and choline. Ethanol is a membrane-disordering agent which has been shown to inhibit synaptosomal high-affinity choline uptake at pharmacologically relevant ethanol concentrations, and to inhibit synaptosomal GABA uptake at higher ethanol concentrations. Dimethylsulfoxide (DMSO) is an organic solvent which has been shown to have a stabilizing effect on artificial phospholipid bilayers, and to have effects on conformation of and cation binding to brain (Na+, K+)-ATPase which are opposite those of ethanol. DMSO alone (2-10% v/v) inhibited synaptosomal uptakes of GABA and of choline in a concentration-dependent fashion, with choline uptake inhibited to a greater degree than GABA uptake. This result is qualitatively similar to the effects of ethanol on these uptake processes. DMSO at low concentrations (0.3-1.5% v/v) had no effect on inhibition of GABA and choline uptake by 0.6 M ethanol, and higher DMSO concentrations resulted only in further inhibition. Similarly, ethanol (0.3 M) had no effect on inhibition of GABA and choline uptake by 5% (v/v) DMSO, and higher ethanol concentrations (0.6-1.2 M) resulted only in further inhibition. We conclude that the inhibiting effects of ethanol on synaptosomal GABA and choline uptake are not reversed by DMSO.
Authors:
P E North; R E Mrak
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurotoxicology     Volume:  10     ISSN:  0161-813X     ISO Abbreviation:  Neurotoxicology     Publication Date:  1989  
Date Detail:
Created Date:  1990-04-12     Completed Date:  1990-04-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7905589     Medline TA:  Neurotoxicology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  569-76     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock.
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MeSH Terms
Descriptor/Qualifier:
Animals
Choline / metabolism*
Dimethyl Sulfoxide / pharmacology*
Ethanol / pharmacology*
Membrane Fluidity / drug effects
Microscopy, Electron
Rats
Rats, Inbred Strains
Synaptosomes / drug effects,  metabolism*,  ultrastructure
gamma-Aminobutyric Acid / metabolism*
Grant Support
ID/Acronym/Agency:
AA06915/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
56-12-2/gamma-Aminobutyric Acid; 62-49-7/Choline; 64-17-5/Ethanol; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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