Document Detail


Synapse loss from chronically elevated glucocorticoids: relationship to neuropil volume and cell number in hippocampal area CA3.
MedLine Citation:
PMID:  16871536     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Individuals with clinical disorders associated with elevated plasma glucocorticoids, such as major depressive disorder and Cushing's syndrome, are reported to have smaller hippocampal volume. To understand how the hippocampus responds at the cellular and subcellular levels to glucocorticoids and how such changes are related to volume measures, we have undertaken a comprehensive study of glucocorticoid effects on hippocampal CA3 volume and identified elements in the neuropil including astrocytic volume and cell and synapse number and size. Male Sprague-Dawley rats were injected with corticosterone (40 mg/kg), the primary glucocorticoid in rodents, or vehicle for 60 days. The CA3 was further subdivided so that the two-thirds of CA3 (nearest the dentate gyrus) previously shown to be vulnerable to corticosterone could be analyzed as two separate subfields. Corticosterone had no effect on neuropil volume or glial volume in the proximal subfield but caused a strong tendency for astrocytic processes to make up a larger proportion of the tissue and for volume of tissue made of constituents other than glial cells (primarily neuronal processes) to be smaller in the middle subfield. Within the neuropil, there were no cellular or subcellular profiles that indicated degeneration, suggesting that corticosterone does not cause prolonged damage. Corticosterone did not reduce cell number or cell or nonperforated synapse size but did cause a pronounced loss of synapses. This loss occurred in both subfields and, therefore, was independent of volume loss. Together, the findings suggest that volume measures can underestimate corticosterone effects on neural structure.
Authors:
Despina A Tata; Veronica A Marciano; Brenda J Anderson
Related Documents :
17525116 - Optical imaging of ca2+-evoked fluid secretion by murine nasal submucosal gland serous ...
11539036 - Influence of microgravity on cellular differentiation in root caps of zea mays.
6812436 - Cell volume regulation in the proximal convoluted tubule.
993066 - Autoradiographic and ultrastructural study of cucurbita pepo root cells during their gr...
3105356 - Comparative study on carbonic anhydrase activity in the retina of different birds durin...
24553126 - Characterization of a bioactive fiber scaffold with entrapped huvecs in coaxial electro...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  498     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-01     Completed Date:  2006-10-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  363-74     Citation Subset:  IM    
Affiliation:
Department of Psychology, State University of New York at Stony Brook, Stony Brook, New York 11790-2500, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / metabolism,  pathology
Atrophy / chemically induced,  physiopathology*
Cell Count
Cell Death / drug effects,  physiology
Chronic Disease
Depressive Disorder, Major / complications,  physiopathology
Disease Models, Animal
Gliosis / chemically induced,  physiopathology
Glucocorticoids / adverse effects,  metabolism*
Hippocampus / metabolism,  pathology*,  physiopathology
Male
Nerve Degeneration / chemically induced,  physiopathology*
Neurons / drug effects,  metabolism,  pathology*
Neuropil / drug effects,  metabolism,  pathology*
Pituitary ACTH Hypersecretion / complications,  physiopathology
Pyramidal Cells / drug effects,  metabolism,  pathology
Rats
Rats, Sprague-Dawley
Stress, Physiological / complications,  physiopathology
Synapses / drug effects,  metabolism,  pathology*
Up-Regulation / physiology
Grant Support
ID/Acronym/Agency:
MH62075/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Glucocorticoids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibitory effect of stobadine on FMLP-induced chemiluminescence in human whole blood and isolated p...
Next Document:  Spatial exploration induces ARC, a plasticity-related immediate-early gene, only in calcium/calmodul...