| Sympathetic nerve stimulation versus pancreatic norepinephrine infusion in the dog: 2). Effects on basal release of somatostatin and pancreatic polypeptide. | |
| | |
MedLine Citation:
|
PMID: 2885175 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We compared the effects of sympathetic nerve stimulation to that of pancreatic norepinephrine (NE) infusion on pancreatic somatostatin-like immunoreactivity (SLI) and pancreatic polypeptide (PP) secretion in the halothane-anesthetized dog. During electrical stimulation (8 Hz, 1 msc, 10 mA, n = 6) of the sympathetic nerves surrounding the pancreatic artery, the pancreatic SLI output decreased from 827 +/- 340 fmol/min to 231 +/- 47 fmol/min (delta = -596 +/- 217 fmol/min, P less than 0.05) after 5 min, and PP output decreased from 11,972 +/- 374 pg/min to 5,518 +/- 774 pg/min (delta = -6,454 +/- 1,932 pg/min, P less than 0.02) after 3 min of stimulation. Arterial SLI or PP levels did not change. Sympathetic nerve stimulation also decreased pancreatic blood flow and increased pancreatic venous NE levels. To determine whether the NE, released locally during sympathetic nerve stimulation, is responsible for this inhibition of pancreatic SLI and PP outputs, exogenous NE was infused into the pancreatic artery at three different dose levels. The low dose of 12 ng/min (n = 6) increased pancreatic venous NE levels like sympathetic nerve stimulation. The medium dose of 120 ng/min (n = 6) reproduced the nerve stimulation-induced decrease of pancreatic blood flow. The high dose of 1,200 ng/min (n = 6) markedly exceeded both. It was found that neither the low nor the medium infusion rates of NE significantly affected pancreatic SLI or PP output. In contrast, infusion of NE at the very high dose level of 1,200 ng/min decreased pancreatic SLI output from 850 +/- 165 fmol/min to 318 +/- 59 fmol/min after 5 min of infusion (delta = -532 +/- 143 fmol/min, P less than 0.01) and decreased PP secretion from 22,777 +/- 7,082 pg/min to 12,764 +/- 6,100 pg/min after 3 min of infusion (delta = -10,013 +/- 2,399 pg/min, P less than 0.01). During this high dose rate NE infusion, both the increment of pancreatic venous SPV levels of NE and the decrement of pancreatic blood flow markedly exceeded the effects produced by sympathetic nerve stimulation. We conclude from this study in dogs: that selective electrical stimulation of the sympathetic nerves entering the pancreas decreases blood flow and inhibits pancreatic SLI and PP secretion, that NE infused into the pancreatic artery at moderate rates reproduces the decrease in blood flow yet does not reproduce the inhibition of pancreatic SLI and PP secretion, and that extremely high concentrations of NE, which markedly restrict pancreatic blood flow, decrease both pancreatic SLI and PP outputs.(ABSTRACT TRUNCATED AT 400 WORDS) |
| | |
Authors:
|
B Ahrén; R C Veith; T L Paquette; G J Taborsky |
Related Documents
:
|
2836225 - Food deprivation decreases the blood trh concentration in the rat. 6202865 - Molecular weight dependence of permselectivity to rat small intestinal blood-lymph barr... 8954315 - Improvement of pancreatic capillary blood flow does not augment the pancreatic tissue c... 1146795 - Cardiovascular lesions in pancreatitis. 2238265 - The use of a diasonics drf400 duplex ultrasound scanner to measure volume flow in arter... 10711365 - The results of intraoperative autotransfusion in orthopaedic surgery. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Endocrinology Volume: 121 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1987 Jul |
Date Detail:
|
Created Date: 1987-07-27 Completed Date: 1987-07-27 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 332-9 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arteries Dogs Electric Stimulation Kinetics Norepinephrine / blood, pharmacology* Pancreas / blood supply, innervation, secretion* Pancreatic Polypeptide / secretion* Regional Blood Flow / drug effects Somatostatin / secretion* Sympathetic Nervous System / physiology* Veins |
| Grant Support | |
ID/Acronym/Agency:
|
AM-1289/AM/NIADDK NIH HHS; AM-17047/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
51-41-2/Norepinephrine; 51110-01-1/Somatostatin; 59763-91-6/Pancreatic Polypeptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Release of dynorphin-like immunoreactivity from rat adenohypophysis in vitro during inhibition of an...
Next Document: The neuropeptide-synthesizing rat 44-2C cell line: regulation of peptide synthesis, secretion, 3,'5'...