| Symmetry breaking and polarization of the C. elegans zygote by the polarity protein PAR-2. | |
| | |
MedLine Citation:
|
PMID: 20392744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Polarization of the C. elegans zygote is initiated by ECT-2-dependent cortical flows, which mobilize the anterior PAR proteins (PAR-3, PAR-6 and PKC-3) away from the future posterior end of the embryo marked by the sperm centrosome. Here, we demonstrate the existence of a second, parallel and redundant pathway that can polarize the zygote in the absence of ECT-2-dependent cortical flows. This second pathway depends on the polarity protein PAR-2. We show that PAR-2 localizes to the cortex nearest the sperm centrosome even in the absence of cortical flows. Once on the cortex, PAR-2 antagonizes PAR-3-dependent recruitment of myosin, creating myosin flows that transport the anterior PAR complex away from PAR-2 in a positive-feedback loop. We propose that polarity in the C. elegans zygote is initiated by redundant ECT-2- and PAR-2-dependent mechanisms that lower PAR-3 levels locally, triggering a positive-feedback loop that polarizes the entire cortex. |
| | |
Authors:
|
Seth Zonies; Fumio Motegi; Yingsong Hao; Geraldine Seydoux |
Related Documents
:
|
1514874 - Regional xenon 133 cerebral blood flow and cerebral technetium 99m hmpao uptake in unme... 10800034 - Assessment of regional cerebral blood flow by dynamic susceptibility contrast mri using... 835154 - Action of vasodilators on regional cerebral blood flow in subacute or chronic cerebral ... 9350404 - Regional cerebral blood flow changes related to affective speech presentation in persis... 15565484 - Cervical spinal cord stimulation increases cerebral cortical blood flow in an experimen... 15365004 - Diagnosing invasive aspergillosis during antifungal therapy by pcr analysis of blood sa... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-04-14 |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 137 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-04-30 Completed Date: 2010-06-02 Revised Date: 2010-11-22 |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
|
Languages: eng Pagination: 1669-77 Citation Subset: IM |
Affiliation:
|
Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Center for Cell Dynamics, Johns Hopkins School of Medicine, PCTB 706, Baltimore, MD 21205, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alleles Amino Acid Sequence Animals Animals, Genetically Modified Body Patterning / genetics* Caenorhabditis elegans / embryology, genetics Caenorhabditis elegans Proteins / genetics, metabolism, physiology* Cell Polarity / genetics* Embryo, Nonmammalian Genes, Helminth / physiology Green Fluorescent Proteins / genetics, metabolism Guanine Nucleotide Exchange Factors / genetics Molecular Sequence Data Sequence Homology, Amino Acid Signal Transduction / genetics, physiology Zygote / growth & development*, metabolism, physiology |
| Grant Support | |
ID/Acronym/Agency:
|
HD37047/HD/NICHD NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
|
0/Caenorhabditis elegans Proteins; 0/Ect-2 protein, C elegans; 0/Guanine Nucleotide Exchange Factors; 0/par-2 protein, C elegans; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebraf...
Next Document: Genomic cis-regulatory networks in the early Ciona intestinalis embryo.