Document Detail

Symbiotic Chlorella sp. of the ciliate Paramecium bursaria do not prevent acidification and lysosomal fusion of host digestive vacuoles during infection.
MedLine Citation:
PMID:  15997335     Owner:  NLM     Status:  MEDLINE    
Each symbiotic Chlorella sp. of the ciliate Paramecium bursaria is enclosed in a perialgal vacuole derived from the host digestive vacuole, and thereby the alga is protected from digestion by lysosomal fusion. Algae-free cells can be reinfected with algae isolated from algae-bearing cells by ingestion into digestive vacuoles. To examine the timing of acidification and lysosomal fusion of the digestive vacuoles and of algal escape from the digestive vacuole, algae-free cells were mixed with isolated algae or yeast cells stained with pH indicator dyes at 25+/-1 degrees C for 1.5 min, washed, chased, and fixed at various time points. Acidification of the vacuoles and digestion of Chlorella sp. began at 0.5 and 2 min after mixing, respectively. All single green Chlorella sp. that had been present in the host cytoplasm before 0.5 h after mixing were digested by 0.5 h. At 1 h after mixing, however, single green algae reappeared in the host cytoplasm, arising from those digestive vacuoles containing both nondigested and partially digested algae, and the percentage of such cells increased to about 40% at 3 h. At 48 h, the single green algae began to multiply by cell division, indicating that these algae had succeeded in establishing endosymbiosis. In contrast to previously published studies, our data show that an alga can successfully escape from the host's digestive vacuole after acidosomal and lysosomal fusion with the vacuole has occurred, in order to produce endosymbiosis.
Yuuki Kodama; Masahiro Fujishima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-07-08
Journal Detail:
Title:  Protoplasma     Volume:  225     ISSN:  0033-183X     ISO Abbreviation:  Protoplasma     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-17     Completed Date:  2005-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  191-203     Citation Subset:  IM    
Biological Institute, Faculty of Science, Yamaguchi University, Yamaguchi, Japan.
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MeSH Terms
Chlorella / metabolism,  physiology*,  ultrastructure
Hydrogen-Ion Concentration
Lysosomes / metabolism,  physiology
Paramecium / metabolism,  physiology*,  ultrastructure
Saccharomyces cerevisiae / physiology
Symbiosis / physiology
Vacuoles / metabolism*,  physiology,  ultrastructure

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