Document Detail


SyM-BBB: a microfluidic Blood Brain Barrier model.
MedLine Citation:
PMID:  23344641     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Current techniques for mimicking the Blood-Brain Barrier (BBB) largely use incubation chambers (Transwell) separated with a filter and matrix coating to represent and to study barrier permeability. These devices have several critical shortcomings: (a) they do not reproduce critical microenvironmental parameters, primarily anatomical size or hemodynamic shear stress, (b) they often do not provide real-time visualization capability, and (c) they require a large amount of consumables. To overcome these limitations, we have developed a microfluidics based Synthetic Microvasculature model of the Blood-Brain Barrier (SyM-BBB). The SyM-BBB platform is comprised of a plastic, disposable and optically clear microfluidic chip with a microcirculation sized two-compartment chamber. The chamber is designed in such a way as to permit the realization of side-by-side apical and basolateral compartments, thereby simplifying fabrication and facilitating integration with standard instrumentation. The individually addressable apical side is seeded with endothelial cells and the basolateral side can support neuronal cells or conditioned media. In the present study, an immortalized Rat Brain Endothelial cell line (RBE4) was cultured in SyM-BBB with a perfusate of Astrocyte Conditioned Media (ACM). Biochemical analysis showed upregulation of tight junction molecules while permeation studies showed an intact BBB. Finally, transporter assay was successfully demonstrated in SyM-BBB indicating a functional model.
Authors:
Balabhaskar Prabhakarpandian; Ming-Che Shen; Joseph B Nichols; Ivy R Mills; Marta Sidoryk-Wegrzynowicz; Michael Aschner; Kapil Pant
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Lab on a chip     Volume:  13     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-20     Completed Date:  2013-08-27     Revised Date:  2014-03-26    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  1093-101     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood-Brain Barrier / metabolism*
Cell Line
Cell Tracking
Culture Media, Conditioned / chemistry
Dextrans / chemistry
Microfluidic Analytical Techniques / instrumentation,  methods*
Models, Biological
P-Glycoprotein / metabolism
Permeability
Rats
Rhodamine 123 / chemistry
Grant Support
ID/Acronym/Agency:
P30 ES000267/ES/NIEHS NIH HHS; R01 ES 07331-16/ES/NIEHS NIH HHS; R01 ES 10563-11/ES/NIEHS NIH HHS; R43GM087129/GM/NIGMS NIH HHS; R44 GM087129/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/P-Glycoprotein; 1N3CZ14C5O/Rhodamine 123; K3R6ZDH4DU/Dextrans
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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