| Sws1 is a conserved regulator of homologous recombination in eukaryotic cells. | |
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MedLine Citation:
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PMID: 16710300 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rad52-dependent homologous recombination (HR) is regulated by the antirecombinase activities of Srs2 and Rqh1/Sgs1 DNA helicases in fission yeast and budding yeast. Functional analysis of Srs2 in Schizosaccharomyces pombe led us to the discovery of Sws1, a novel HR protein with a SWIM-type Zn finger. Inactivation of Sws1 suppresses the genotoxic sensitivity of srs2Delta and rqh1Delta mutants and rescues the inviability of srs2Delta rqh1Delta cells. Sws1 functions at an early step of recombination in a pro-recombinogenic complex with Rlp1 and Rdl1, two RecA-like proteins that are most closely related to the human Rad51 paralogs XRCC2 and RAD51D, respectively. This finding indicates that the XRCC2-RAD51D complex is conserved in lower eukaryotes. A SWS1 homolog exists in human cells. It associates with RAD51D and ablating its expression reduces the number of RAD51 foci. These studies unveil a conserved pathway for the initiation and control of HR in eukaryotic cells. |
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Authors:
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Victoria Martín; Charly Chahwan; Hui Gao; Véronique Blais; James Wohlschlegel; John R Yates; Clare H McGowan; Paul Russell |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-05-18 |
Journal Detail:
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Title: The EMBO journal Volume: 25 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-07 Completed Date: 2006-08-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
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Languages: eng Pagination: 2564-74 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals DNA Helicases / genetics, metabolism* Epistasis, Genetic Humans Molecular Sequence Data Rad51 Recombinase / genetics, metabolism Recombination, Genetic* Saccharomyces cerevisiae Proteins / genetics, metabolism Schizosaccharomyces / genetics*, metabolism Schizosaccharomyces pombe Proteins / genetics, metabolism* Sequence Alignment Sequence Homology, Amino Acid Two-Hybrid System Techniques Zinc Fingers |
| Grant Support | |
ID/Acronym/Agency:
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CA77325/CA/NCI NIH HHS; EY1328801/EY/NEI NIH HHS; GM59447/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RHP51 protein, S pombe; 0/Saccharomyces cerevisiae Proteins; 0/Schizosaccharomyces pombe Proteins; 0/Sws1 protein, S pombe; EC 2.7.7.-/Rad51 Recombinase; EC 3.6.1.-/DNA Helicases; EC 5.99.-/Srs2 protein, S pombe |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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