Document Detail


Switching of chromatin-remodelling complexes for oestrogen receptor-alpha.
MedLine Citation:
PMID:  18451880     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the cell-cycle-dependent associations of these complexes are poorly understood. By using a synchronization system, we found that the transactivation function of ERalpha at G2/M was lowered. Biochemical approaches showed that ERalpha associated with two discrete classes of ATP-dependent chromatin-remodelling complex in a cell-cycle-dependent manner. The components of the NuRD-type complex were identified as G2/M-phase-specific ERalpha co-repressors. Thus, our results indicate that the transactivation function of ERalpha is cell-cycle dependent and is coupled with a cell-cycle-dependent association of chromatin-remodelling complexes.
Authors:
Maiko Okada; Shin-ichiro Takezawa; Yoshihiro Mezaki; Ikuko Yamaoka; Ichiro Takada; Hirochika Kitagawa; Shigeaki Kato
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-02
Journal Detail:
Title:  EMBO reports     Volume:  9     ISSN:  1469-3178     ISO Abbreviation:  EMBO Rep.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-02     Completed Date:  2008-09-17     Revised Date:  2011-04-21    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  563-8     Citation Subset:  IM    
Affiliation:
The Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / physiology*
Cell Line
Chromatin / metabolism*
Chromatin Assembly and Disassembly*
Estrogen Receptor alpha / genetics,  metabolism*
Estrogens / metabolism
Female
Humans
Macromolecular Substances / metabolism*
Transcriptional Activation
Chemical
Reg. No./Substance:
0/Chromatin; 0/Estrogen Receptor alpha; 0/Estrogens; 0/Macromolecular Substances; 0/estrogen receptor alpha, human
Comments/Corrections

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