Document Detail


Swim-Exercised Mice Show a Decreased Level of Protein O-GlcNAcylation and Expression of O-GlcNAc Transferase in Heart.
MedLine Citation:
PMID:  21493720     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Swim training exercise in mice leads to cardiac remodeling associated with an improvement in contractile function. Protein O-GlcNAcylation is a post-translational modification of serine and threonine residues capable of altering protein-protein interactions affecting gene transcription, cell signaling pathways and general cell physiology. Increased levels of protein O-GlcNAcylation in the heart have been associated with pathological conditions such as diabetes, ischemia and hypertrophic heart failure. In contrast, the impact of physiological exercise on protein O-GlcNAcylation in the heart is currently unknown. Swim training exercise in mice was associated with the development of a physiological hypertrophy characterized by an improvement in contractile function relative to sedentary mice. General protein O-GlcNAcylation was significantly decreased in swim-exercised mice. This effect was mirrored in the level of O-GlcNAcylation of individual proteins such as SP1. The decrease in protein O-GlcNAcylation was associated with a decrease in the expression of O-GlcNAc transferase (OGT) and GFAT2 mRNA. O-GlcNAcase (OGA) activity was actually lower in swim trained than sedentary hearts, suggesting that it did not contribute to the decreased protein O-GlcNAcylation. Thus, it appears that exercise-induced physiological hypertrophy is associated with a decrease in protein O-GlcNAcylation, which could potentially contribute to changes in gene expression and other physiological changes associated with exercise.
Authors:
Darrell D Belke
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-14
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  -     ISSN:  1522-1601     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1University of Calgary.
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