Document Detail


Swelling and replicative DNA synthesis of detergent-treated mouse ascites sarcoma cells.
MedLine Citation:
PMID:  148832     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous investigation showed that mouse ascites sarcoma cells permeabilized with appropriate concentrations of detergents (Triton X-100, Nonidet P-40 and Brij 58) had high replicative DNA synthesis in the presence of the four deoxyribonucleoside triphosphates, ATP, Mg2+ and proper ionic environment. The present study showed the optimum detergent concentration for DNA synthesis coincided closely with the minimum detergent concentration for inducing cell swelling. Phase contrast microscopy and electron microscopy of Triton-permeabilized cells showed the characteristic swollen cytoplasms and nucleus. Autoradiographic study showed that the DNA synthesis in permeable cells was confined to the nucleus. Cell viability and [3H] deoxythymidine uptake were impaired at much lower concentrations of Triton X-100 than the optimum concentration for in vitro DNA synthesis. In Triton-permeabilized cells, the minimum Triton concentration that produced cell swelling also seemed to produce high repliative DNA synthesis, which reflects the in vivo state of DNA synthesis.
Authors:
S Seki; T Nakamura; K Kataoka; T Oda
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta medica Okayama     Volume:  32     ISSN:  0386-300X     ISO Abbreviation:  Acta Med. Okayama     Publication Date:  1978 Apr 
Date Detail:
Created Date:  1978-07-24     Completed Date:  1978-07-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0417611     Medline TA:  Acta Med Okayama     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / drug effects
Cells, Cultured
DNA Replication*
DNA, Neoplasm / biosynthesis*
Detergents / pharmacology*
Mice
Polyethylene Glycols / pharmacology
Sarcoma, Experimental / metabolism*
Thymidine / metabolism
Thymine Nucleotides / metabolism
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Detergents; 0/Polyethylene Glycols; 0/Thymine Nucleotides; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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