| Swelling rather than shrinkage precedes apoptosis in serum-deprived vascular smooth muscle cells. | |
| | |
MedLine Citation:
|
PMID: 22249286 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Contrasting cell volume behaviours (swelling vs. shrinkage) are considered as criteria to distinguish necrosis from apoptosis. In this study, we employed a time-lapse, dual-image surface reconstruction technique to assess the volume of single vascular smooth muscle cells transfected with E1A-adenoviral protein (E1A-VSMC) and undergoing rapid apoptosis in the absence of growth factors or in the presence of staurosporine. After 30- to 60-min lag-phase, serum-deprived E1A-VSMC volume was increased by ~40%, which preceded maximal increments of caspase-3 activity and chromatin cleavage. Swollen cells underwent rapid apoptotic collapse, documented by plasma membrane budding, and terminated in 10-15 min by the formation of numerous apoptotic bodies. Suppression of apoptosis by inhibition of Na(+),K(+)-ATPase and activation of cAMP signalling with ouabain and forskolin, respectively, completely abolished the swelling of serum-deprived E1A-VSMC. In contrast to serum deprivation, apoptotic collapse of staurosporine-treated E1A-VSMC preceded attenuation of their volume by ~30%. Neither transient hyposmotic swelling nor isosmtotic shrinkage triggered apoptosis. Our results show that cell shrinkage can not be considered as ubiquitous hallmark of apoptosis. The involvement of stimulus-specific cell volume perturbations in initiation and progression of apoptosis in vascular smooth muscle cells should be examined further. |
| | |
Authors:
|
Alexandra Platonova; Svetlana V Koltsova; Pavel Hamet; Ryszard Grygorczyk; Sergei N Orlov |
Related Documents
:
|
9688186 - Macrophages in lung tissue from patients with pulmonary emphysema express both inducibl... 8575936 - Nitric oxide in physiology and pathology. 9845346 - Transcellular regulation of cell respiration by nitric oxide generated by activated mac... 7986156 - Nitric oxide inhibition normalizes splenocyte interleukin-10 synthesis in murine therma... 1100536 - Dose titration of steroidal and non-steroidal topical anti-inflammatory agents. 14687706 - Transcription and translation of the chemokines rantes and mcp-1 in nasal polyps and mu... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-17 |
Journal Detail:
|
Title: Apoptosis : an international journal on programmed cell death Volume: - ISSN: 1573-675X ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Research Centre, Centre hospitalier de l'Université de Montréal (CRCHUM), Technopôle Angus, 2901 Rachel East, Montreal, QC, H1W 4A4, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hepatitis B vaccine induces apoptotic death in Hepa1-6 cells.
Next Document: Association of CTLA-4 gene polymorphisms with sporadic breast cancer risk and clinical features in H...