Document Detail


Swelling and pressure-volume relationships in the dermis measured by osmotic-stress technique.
MedLine Citation:
PMID:  19321700     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Water transfer across the extracellular matrix (ECM) involves interstitial osmotic forces in as yet unclear ways. In particular, the traditional values of Starling forces cannot adequately explain fluid transfer rates. Here, we reassess these forces by analyzing fluid transfer in live pig and human dermal explants. Pressure potentials were controlled with inert polymers adjusted by membrane osmometry (range = 3-219 mmHg), and fluid transfer in and out of the explants was followed by sequential precision weighing. Water motional freedom in the dermis was examined by NMR. In pigs, mean hydration pressure (HP; the pressure at which volume did not change) was 107 +/- 22 and 47 +/- 12 (SE) mmHg at 4 degrees C and 37 degrees C (P = 0.012, paired t-test, n = 7). Volume changes observed in response to pressure potential were reversible. The equation, Volume change = V(max)/[1+(time/T(1/2))(d)], where V(max) is maximal volume change; T(1/2), time at volume = 1/2 V(max); and d, a rate parameter, was fitted to experimental progression curves (r(2) > 0.9), yielding V(max) values linearly related to pressure, with mean slopes -3.5 +/- 0.28 and -2.6 +/- 0.21(SE) mul.g(-1).mmHg(-1) at 4 degrees C and 37 degrees C. NMR spin-spin relaxation times (T(2)) varied within 200- to 400-mum distances in directions perpendicular to the epidermis, with slopes reaching 0.03 ms/mum. Results support a mechanism in which fluid transport across the ECM is locally regulated at micrometer scales by cell- and fiber-gel-dependent osmomechanical forces. The large HP helps to explain the fast interstitial in/out flow rates observed clinically.
Authors:
Maria P McGee; Michael Morykwas; Nicole Levi-Polyachenko; Louis Argenta
Publication Detail:
Type:  Journal Article     Date:  2009-03-25
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  296     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-21     Completed Date:  2009-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1907-13     Citation Subset:  IM    
Affiliation:
Plastic and Reconstructive Surgery Research, Surgery Division, Wake-Forest Univeristy Medical School, Medical Center Blvd., Winston-Salem, NC 27157, USA. mmcgee@wfubmc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Size*
Dermis / metabolism*
Extracellular Matrix / metabolism*
Humans
Kinetics
Magnetic Resonance Spectroscopy
Mechanotransduction, Cellular*
Models, Biological
Osmotic Pressure
Swine
Temperature
Water / metabolism*
Chemical
Reg. No./Substance:
7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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