| Swelling-activated chloride currents in embryonic chick heart cells. | |
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MedLine Citation:
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PMID: 11501066 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To characterize a swelling-activated chloride current, I(Cl, swell), in white Leghorn chick heart cells and the effects of chlorpromazine (CPZ) effects. METHODS: The patch-clamp technique in the whole-cell configuration was used. RESULTS: Hyposmotic swelling elicited I(Cl, swell) in white Leghorn chick heart cells. The current amplitude increased from (452 +/- 200) pA to (849 +/- 373) pA with a reduction of osmolarity from 300 mmol.L-1 to 270 mmol.L-1, 4',4-Diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) 100 mumol.L-1 decreased I(Cl, swell) from (1196 +/- 505) pA to (830 +/- 328) pA in hyposmotic solution. In white Leghorn chick heart cells I(Cl, swell) was not induced by CPZ 30 mumol.L-1, which is different from the case of E coli spheroplast. CONCLUSION: Swelling-activated chloride current was activated by hyposmotic swelling in white Leghorn chick heart cells. The mechanism for activating the current is different from that of mechanosensitive ion channels of E coli. |
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Authors:
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H Wei; Y A Mei; M M Wu; J T Sun; H Q Zhou; Z H Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 21 ISSN: 1671-4083 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2001-08-14 Completed Date: 2001-10-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: China |
Other Details:
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Languages: eng Pagination: 986-90 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, Liren Laboratory, Fudan University, Shanghai 200433, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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pharmacology Animals Cells, Cultured Chick Embryo Chloride Channels / drug effects* Chlorpromazine / pharmacology* Heart / physiology Myocardium / cytology* Patch-Clamp Techniques |
| Chemical | |
Reg. No./Substance:
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0/Chloride Channels; 50-53-3/Chlorpromazine; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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