Document Detail


Sutureless colonic anastomosis in the rat: a randomized controlled study.
MedLine Citation:
PMID:  12525906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present study was to evaluate the effect of glue tissue on the healing of colonic anastomosis in rats. Two groups of 20 Wistar rats each were used. Following laparotomy, a segment of 1 cm of the colon was resected, 10 cm from the ileocecal valve. In the control group, the anastomosis was sutured in a single layer with 6-0 polypropylene interrupted extramucosal sutures. In the glue group, the anastomosis was performed by using 2-octyl cyanoacrylate (Dermabond, Ethicon). Rats were sacrificed on day 7 following operation. Integrity of the anastomoses, existence of perianastomotic abscess or peritonitis, and adhesion formation were recorded. Anastomoses were resected including a 2.5-cm of bowel on either side. Bursting pressures were measured and the specimens were sent for histological examination. Anastomotic dehiscence occured in 20% of the animals in each group. Adhesion formation was more extensive in the glue group compared to the control group, but this difference was not statistically significant (p=0.074). Bursting pressures of the anastomoses between the two groups were not statistically significant (p=0.897). The wound healing process, as assessed by inflammatory cell infiltration, blood vessel neodevelopment, collagen deposition and fibroblast activity, did not differ statistically between the two groups (p>0.05). In conclusion, 2-octyl cyanoacrylate provides, under experimental conditions, a sutureless anastomosis equal in healing to the conventionally sutured one. The outcome may differ under demanding clinical situations.
Authors:
I Kanellos; I Mantzoros; H Demetriades; S Kalfadis; L Sakkas; T Kelpis; D Betsis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Techniques in coloproctology     Volume:  6     ISSN:  1123-6337     ISO Abbreviation:  Tech Coloproctol     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2003-01-14     Completed Date:  2003-04-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9613614     Medline TA:  Tech Coloproctol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  143-6     Citation Subset:  IM    
Affiliation:
Fourth Department of Surgery, Aristotle University, Antheon 1, Panorama, GR-55236 Thessaloniki, Greece. ik@hol.gr
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MeSH Terms
Descriptor/Qualifier:
Anastomosis, Surgical / methods*
Animals
Colectomy / methods*
Colon / drug effects,  physiopathology,  surgery*
Colonic Diseases / physiopathology,  surgery*
Control Groups
Cyanoacrylates / therapeutic use*
Disease Models, Animal
Random Allocation
Rats
Rats, Wistar
Suture Techniques*
Tissue Adhesives / therapeutic use*
Treatment Outcome
Wound Healing / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Cyanoacrylates; 0/Tissue Adhesives; 6701-17-3/octyl 2-cyanoacrylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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