Document Detail


Sustained local delivery of heparin to the rabbit arterial wall with an electroporation catheter.
MedLine Citation:
PMID:  9829901     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Current methods of local drug delivery frequently fail to achieve a prolonged therapeutically effective tissue drug level without producing vascular trauma. A novel double-balloon catheter system, incorporating electroporation technology, has been designed and tested to deliver heparin into rabbit carotid arteries in an overstretch balloon injury model in vivo. Following arterial injury, fluoresceinated heparin was delivered into the volume between the two inflated balloons, and the artery was subjected to an electrical pulse. Catheter deployment and endovascular electrical pulsing were well-tolerated in all animals (N = 21) without adverse hemodynamic and histological changes. Periodic arterial blood samples revealed no abnormalities in the clotting profile or any gross morphological changes in the blood cells up to 8 hr after treatment. Much stronger heparin fluorescence was detected throughout the vessel layers for at least 12 hr in the pulsed samples compared to the control. Histochemical staining of the tissue showed intracellular localization of heparin. Endovascular electroporation may provide better retention and higher therapeutic efficacy than can be achieved by conventional systemic delivery of heparin at clinically safe concentrations.
Authors:
N B Dev; T J Preminger; G A Hofmann; S B Dev
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Catheterization and cardiovascular diagnosis     Volume:  45     ISSN:  0098-6569     ISO Abbreviation:  Cathet Cardiovasc Diagn     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-01-20     Completed Date:  1999-01-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7508512     Medline TA:  Cathet Cardiovasc Diagn     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  337-45     Citation Subset:  IM    
Affiliation:
Division of Pediatric Cardiology, Case Western Reserve University, Cleveland, Ohio, USA. nbdev@genetronics.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Anticoagulants / administration & dosage*,  pharmacokinetics
Blood Pressure
Carotid Artery, Common / drug effects,  metabolism,  pathology
Carotid Stenosis / drug therapy,  metabolism,  pathology
Catheterization, Peripheral / instrumentation*
Disease Models, Animal
Drug Delivery Systems / instrumentation*
Electroporation*
Female
Heparin / administration & dosage*,  pharmacokinetics
Injections, Intra-Arterial
Male
Rabbits
Safety
Treatment Outcome
Chemical
Reg. No./Substance:
0/Anticoagulants; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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