Document Detail


Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5/Pho85 family.
MedLine Citation:
PMID:  17405809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cyclin-dependent kinases from the Cdk5/Pho85 family are thought to play important roles in morphogenesis in organisms as diverse as yeast and humans. Here we used the corn smut fungus Ustilago maydis to address the role of Cdk5/Pho85 kinases in the morphogenesis and virulence of dimorphic phytopathogens. We found that Cdk5 is essential for growth in U. maydis. A temperature-sensitive cdk5 mutant caused cell wall and morphology defects at the restrictive temperature. Actin patches labeled with a fimbrin-GFP fusion protein were delocalized and a GFP-Myo5 fusion was directed towards the growing cell pole and rapidly dissociated from the tip. These defects were found to be due to an impairment in the maintenance of cell polarity. Our results indicated that Cdk5 is required for the activity of Rac1, probably at the level of the localization of its GEF, Cdc24. Cdk5 was required for full virulence, probably because mutant cells are unable to sustain the dramatic polar growth required for the formation of the infective structures. These results support a major role for morphogenesis in the virulence program of dimorphic fungi.
Authors:
Sonia Castillo-Lluva; Isabel Alvarez-Tabarés; Isabella Weber; Gero Steinberg; José Pérez-Martín
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-03
Journal Detail:
Title:  Journal of cell science     Volume:  120     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-24     Completed Date:  2007-10-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1584-95     Citation Subset:  IM    
Affiliation:
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología CSIC, 28049 Madrid, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EF190891
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Cell Cycle Proteins / genetics,  metabolism
Cell Division / genetics,  physiology
Cell Nucleus / metabolism
Cell Wall / metabolism
Chitin / analysis,  metabolism
Cyclin-Dependent Kinase 5 / genetics,  metabolism*
Cytoplasm / metabolism
Fungal Proteins / genetics,  metabolism*
G2 Phase / genetics,  physiology
Green Fluorescent Proteins / genetics,  metabolism
Membrane Glycoproteins / metabolism
Microfilament Proteins / metabolism
Microscopy, Fluorescence
Mitogen-Activated Protein Kinase Kinases / genetics,  metabolism
Molecular Sequence Data
Mutation
Myosin Type V / metabolism
Phylogeny
Recombinant Fusion Proteins / genetics,  metabolism
Ustilago / genetics,  metabolism*,  pathogenicity
Virulence
Zea mays / microbiology
rac1 GTP-Binding Protein / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Actins; 0/Cell Cycle Proteins; 0/Fungal Proteins; 0/Membrane Glycoproteins; 0/Microfilament Proteins; 0/Recombinant Fusion Proteins; 0/plastin; 1398-61-4/Chitin; 147336-22-9/Green Fluorescent Proteins; EC 2.7.10.-/Fuz7 protein, Ustilago maydis; EC 2.7.11.22/Cyclin-Dependent Kinase 5; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases; EC 3.6.1.-/Myosin Type V; EC 3.6.5.2/rac1 GTP-Binding Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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