| Suspended animation extends survival limits of Caenorhabditis elegans and Saccharomyces cerevisiae at low temperature. | |
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MedLine Citation:
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PMID: 20462960 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The orderly progression through the cell division cycle is of paramount importance to all organisms, as improper progression through the cycle could result in defects with grave consequences. Previously, our lab has shown that model eukaryotes such as Saccharomyces cerevisiae, Caenorhabditis elegans, and Danio rerio all retain high viability after prolonged arrest in a state of anoxia-induced suspended animation, implying that in such a state, progression through the cell division cycle is reversibly arrested in an orderly manner. Here, we show that S. cerevisiae (both wild-type and several cold-sensitive strains) and C. elegans embryos exhibit a dramatic decrease in viability that is associated with dysregulation of the cell cycle when exposed to low temperatures. Further, we find that when the yeast or worms are first transitioned into a state of anoxia-induced suspended animation before cold exposure, the associated cold-induced viability defects are largely abrogated. We present evidence that by imposing an anoxia-induced reversible arrest of the cell cycle, the cells are prevented from engaging in aberrant cell cycle events in the cold, thus allowing the organisms to avoid the lethality that would have occurred in a cold, oxygenated environment. |
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Authors:
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Kin Chan; Jesse P Goldmark; Mark B Roth |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-05-12 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 21 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-30 Completed Date: 2010-12-16 Revised Date: 2011-03-18 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2161-71 Citation Subset: IM |
Affiliation:
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Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia / metabolism Benomyl / pharmacology Caenorhabditis elegans / cytology, drug effects, embryology, physiology* Caenorhabditis elegans Proteins / genetics, metabolism Cell Cycle / physiology* Cell Shape Cold Temperature* Hibernation / physiology* Microtubule-Organizing Center / metabolism Mutation Nuclear Pore Complex Proteins / genetics, metabolism Oxygen / metabolism Saccharomyces cerevisiae / cytology, drug effects, physiology* Saccharomyces cerevisiae Proteins / genetics, metabolism Survival Rate* Tubulin Modulators / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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R01GM48435/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Caenorhabditis elegans Proteins; 0/NDC1 protein, S cerevisiae; 0/Nuclear Pore Complex Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Tubulin Modulators; 17804-35-2/Benomyl; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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