Document Detail

Susceptibility to vertical transmission of Toxoplasma gondii is temporally dependent on the preconceptional infection in Calomys callosus.
MedLine Citation:
PMID:  17182099     Owner:  NLM     Status:  MEDLINE    
Toxoplasma gondii is an obligate intracellular parasite that causes a variety of clinical syndromes, but the infection is more severe in immunocompromised individuals and in cases of congenital toxoplasmosis. This study aimed to verify if the susceptibility to vertical transmission of Toxoplasma gondii is temporally dependent on the preconceptional infection in Calomys callosus. Twelve C. callosus females were infected with 20 cysts of T. gondii ME49 strain and divided into three groups of four animals that were mated after approximately 10 days (group 1), 30 days (group 2), and 50 days (group 3) of infection. The animals were sacrificed from the 17th to 20th day of pregnancy, when placentas and embryos were collected for morphological and immunohistochemical studies, mouse bioassay for evaluating seroconversion and PCR for detecting parasite DNA. Serum samples from C. callosus females and mice used in bioassay were analysed for the detection of IgG antibodies to T. gondii by ELISA. Detection of T. gondii was observed by mouse bioassay and PCR in placentas and embryos from C. callosus females infected around 10 days pre-conception. However, only placentas, but not embryos, from females infected around 30 and 50 days pre-conception showed positivity for parasite DNA and seroconversion by mouse bioassay. In conclusion, this study model shows that vertical transmission of T. gondii may take place when maternal infection occurs within one month before conception, thus demonstrating the time of preconceptional seroconversion that rule out a risk of congenital toxoplasmosis.
B F Barbosa; D A O Silva; I N Costa; J D O Pena; J R Mineo; E A V Ferro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-19
Journal Detail:
Title:  Placenta     Volume:  28     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-11     Completed Date:  2007-08-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  624-30     Citation Subset:  IM    
Laboratory of Histology and Embriology, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia 38405-320, MG, Brazil.
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MeSH Terms
Antibodies, Protozoan / blood
DNA, Protozoan / analysis
Disease Susceptibility
Infectious Disease Transmission, Vertical*
Placenta / chemistry,  parasitology*
Pregnancy Complications, Parasitic / parasitology*
Toxoplasma / immunology
Toxoplasmosis, Animal / transmission*
Reg. No./Substance:
0/Antibodies, Protozoan; 0/DNA, Protozoan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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