| Susceptibility to hyperosmotic stress-induced phosphatidylserine exposure increases during red blood cell storage. | |
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MedLine Citation:
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PMID: 21077907 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: During storage of red blood cell (RBCs) before transfusion, RBCs undergo a series of structural and functional changes that include the exposure of phosphatidylserine (PS), a potent removal signal. It was postulated that, during blood bank storage, the susceptibility to stress-induced PS exposure increases, thereby rendering a considerable fraction of the RBCs susceptible to rapid removal after transfusion. STUDY DESIGN AND METHODS: RBCs were processed and stored following standard Dutch blood bank procedures. Samples were taken every week for up to 6 weeks and exposed to various stress conditions, such as hyperosmotic shock and energy depletion. The effect of these treatments on PS exposure was measured by flow cytometric analysis of annexin V binding. The same analyses were performed on RBCs that had been separated according to density using discontinuous Percoll gradients. RESULTS: During storage under blood bank conditions, RBCs become increasingly susceptible to loss of phospholipid asymmetry induced by hyperosmotic shock and energy depletion. Especially the RBCs of higher densities, that have a smaller volume and an increased HbA1c content as is typical of aged RBCs, become increasingly susceptible with storage time. CONCLUSIONS: During storage, RBCs develop an increased susceptibility to stress-induced loss of phospholipid asymmetry that is especially associated with an aging phenotype. This increased susceptibility may be responsible for the rapid disappearance of a considerable fraction of the RBCs during the first 24 hours after transfusion. |
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Authors:
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Giel J C G M Bosman; Judith C A Cluitmans; Yvonne A M Groenen; Jan M Werre; Frans L A Willekens; Vĕra M J Novotný |
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Publication Detail:
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Type: Journal Article Date: 2010-11-15 |
Journal Detail:
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Title: Transfusion Volume: 51 ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
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Languages: eng Pagination: 1072-8 Citation Subset: IM |
Copyright Information:
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© 2010 American Association of Blood Banks. |
Affiliation:
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From the Department of Biochemistry and Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen; the Department of Clinical Chemistry, Rijnstate Hospital, Arnhem; and the Department of Hematology, Radboud University Nijmegen Medical Centre and Nijmegen Institute for Infection, Inflammationa and Immunity, Nijmegen, the Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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