Document Detail

Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study.
MedLine Citation:
PMID:  15983029     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The purpose of this study was to evaluate the antimicrobial effect of toluidine blue O (TBO), in combination with either a helium/neon (HeNe) laser or a light-emitting diode (LED), on the viability and architecture of Streptococcus mutans biofilms. METHODS: Biofilms were grown on hydroxyapatite discs in a constant depth film fermentor fed with artificial saliva that was supplemented with 2% sucrose four times a day, thus producing a typical 'Stephan pH curve'. Photodynamic therapy was subsequently carried out on biofilms of various ages with light from either the HeNe laser or LED using energy densities of between 49 and 294 J/cm(2). RESULTS: Significant decreases in the viability of S. mutans biofilms were only observed when biofilms were exposed to both TBO and light, when reductions in viability of up to 99.99% were observed with both light sources. Overall, the results showed that the bactericidal effect was light dose-dependent and that older biofilms were less susceptible to photodynamic therapy. Confocal laser scanning microscopy images suggested that lethal photosensitization occurred predominantly in the outermost layers of the biofilms. CONCLUSIONS: Photodynamic therapy may be a useful approach in the treatment of dental plaque-related diseases.
Iriana Carla Junqueira Zanin; Reginaldo Bruno Gonçalves; Aldo Brugnera Junior; Christopher Keith Hope; Jonathan Pratten
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-06-27
Journal Detail:
Title:  The Journal of antimicrobial chemotherapy     Volume:  56     ISSN:  0305-7453     ISO Abbreviation:  J. Antimicrob. Chemother.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-01     Completed Date:  2005-09-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7513617     Medline TA:  J Antimicrob Chemother     Country:  England    
Other Details:
Languages:  eng     Pagination:  324-30     Citation Subset:  IM    
Department of Microbiology and Immunology, Dental School of Piracicaba, State University of Campinas, 901 Limeira Av., Piracicaba, São Paulo, Brazil.
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MeSH Terms
Biofilms / drug effects,  growth & development,  radiation effects*
Dose-Response Relationship, Radiation
Hydrogen-Ion Concentration
Laser Therapy
Microscopy, Confocal
Phototherapy / methods*
Streptococcus mutans / drug effects,  growth & development,  radiation effects*
Tolonium Chloride / pharmacology
Reg. No./Substance:
92-31-9/Tolonium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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