Document Detail


Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study.
MedLine Citation:
PMID:  19585079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of Staphylococcus aureus to develop multidrug resistance is well documented, and the antibiotic resistance showed by an increasing number of bacteria has shown the need for alternative therapies to treat infections, photodynamic therapy (PDT) being a potential candidate. The aim of this study was to determine the effect of photodynamic therapy as a light-based bactericidal modality to eliminate Staphylococcus aureus. The study investigated a technique based on a combination of light and a photosensitizer that is capable of producing oxidative species to induce a cytotoxic effect. A Staphylococcus aureus suspension was exposed to a light emitting diode (LED) emitting at 628 nm, 14.6 mW/cm(2), and energy density of 20 J/cm(2), 40 J/cm(2), or 60 J/cm(2) in the presence of different porphyrin concentrations (Photogem). Three drug concentrations were employed: 12 microl/ml, 25 microl/ml, and 50 microl/ml. The treatment response was evaluated by the number of bacterial colony forming units (CFU) after light exposure. The results indicated that exposure to 60 J/cm(2) eliminated 100% (10 log(10) scales) of bacteria, on average. The best PDT response rate to eliminate Staphylococcus aureus was achieved with exposure to LED light in combination with the photosensitizer at concentrations ranging from 25 microl/ml to 50 microl/ml. These data suggest that PDT has the potential to eliminate Staphylococcus aureus in suspension and indicates the necessary drug concentration and light fluency.
Authors:
Mariana M Gois; Christina Kurachi; E J B Santana; E G O Mima; D M P Spolid?rio; Jos? Eduardo Pelizon Pelino; Vanderlei Salvador Bagnato
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Publication Detail:
Type:  Evaluation Studies; In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-08
Journal Detail:
Title:  Lasers in medical science     Volume:  25     ISSN:  1435-604X     ISO Abbreviation:  Lasers Med Sci     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-09     Completed Date:  2010-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8611515     Medline TA:  Lasers Med Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  391-5     Citation Subset:  IM    
Affiliation:
Araraquara School of Dentistry, S?o Paulo State University (UNESP), Araraquara, S?o Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial
Hematoporphyrins / pharmacology*
Humans
Lasers, Semiconductor
Photochemotherapy*
Photosensitizing Agents / pharmacology*
Staphylococcal Infections / drug therapy
Staphylococcus aureus / drug effects*,  radiation effects
Chemical
Reg. No./Substance:
0/Hematoporphyrins; 0/Photosensitizing Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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