| Susceptibility of Legionella species to five antibiotics and development of resistance by exposure to erythromycin, ciprofloxacin, and rifampicin. | |
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MedLine Citation:
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PMID: 10744366 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The minimal inhibitory concentration (MIC) values of erythromycin, ciprofloxacin, ofloxacin, rifampicin, and clindamycin were determined for 56 strains of Legionella pneumophila (38 patient, 3 environmental, and 15 reference strains) and 37 strains of other Legionella species (7 patient, 2 environmental, and 28 reference strains) using the epsilon-test system on BCYEalpha agar plates. High-level resistance (MIC > or = 4 microg/mL) was found only for clindamycin (57%), with MIC values ranging from 0.25-32 microg/mL. Low-level resistance was found for erythromycin (18%) (0.5 < MIC < 8), ciprofloxacin (1%) (1 < MIC < 4), and clindamycin (40%) (0.5 < MIC < 4), but not for ofloxacin and rifampicin. MIC50 for the 45 Danish clinical Legionella strains were 0.25 microg/mL (erythromycin), 0.25 microg/mL (ciprofloxacin), 0.19 microg/mL (ofloxacin), below 0.016 microg/mL (rifampicin), and 4 microg/mL (clindamycin). Of the clinical isolates, 64% were resistant to clindamycin. There were no significant differences between the MIC50 values obtained for clinical and nonclinical Legionella strains. Selected susceptible strains were exposed to increasing concentrations of either erythromycin, ciprofloxacin, or rifampicin to select for resistance. Isolates resistant to erythromycin (MIC 0.75-32 microg/mL) or ciprofloxacin (MIC 2-3 microg/mL) could be selected by a two-step procedure. One single strain recovered from media containing 50 microg/mL of erythromycin had an MIC value higher than 256 microg/mL to erythromycin. In contrast, high-level resistance toward rifampicin with MIC > or = 256 microg/mL developed as a one-step phenomenon in several strains. |
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Authors:
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K Nielsen; J M Bangsborg; N Høiby |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Diagnostic microbiology and infectious disease Volume: 36 ISSN: 0732-8893 ISO Abbreviation: Diagn. Microbiol. Infect. Dis. Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-05-05 Completed Date: 2000-05-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8305899 Medline TA: Diagn Microbiol Infect Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 43-8 Citation Subset: IM |
Affiliation:
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Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology Anti-Infective Agents / pharmacology Antibiotics, Antitubercular / pharmacology Ciprofloxacin / pharmacology* Drug Resistance, Multiple / physiology* Erythromycin / pharmacology* Humans Legionella / classification*, drug effects* Microbial Sensitivity Tests Reproducibility of Results Rifampin / pharmacology* Sensitivity and Specificity Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Anti-Infective Agents; 0/Antibiotics, Antitubercular; 114-07-8/Erythromycin; 13292-46-1/Rifampin; 85721-33-1/Ciprofloxacin |
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