Document Detail


Susceptibility of Escherichia coli to bactericidal action of lactoperoxidase, peroxide, and iodide or thiocyanate.
MedLine Citation:
PMID:  348097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bactericidal action that results from lactoperoxidase-catalyzed oxidation of iodide or thiocyanate was studied, using Escherichia coli as the test organism. The susceptibility of intact cells to bactericidal action was compared with that of cells with altered cell envelopes. Exposure to ethylenediaminetetraacetic acid, to lysozyme and ethylenediaminetetraacetic acid, or to osmotic shock were used to alter the cell envelope. Bactericidal action was greatly increased when the cells were exposed to the lactoperoxidase-peroxide-iodide system at low temperatures, low cell density, or after alteration of the cell envelope. When thiocyanate was substituted for iodide, bactericidal activity was observed only at low cell density or after osmotic shock. Low temperature and low cell density lowered the rate of destruction of peroxide by the bacteria. Therefore, competition for peroxide between the bacteria and lactoperoxidase may influence the extent of bactericidal action. Alteration of the cell envelope had only a small effect on the rate of destruction of peroxide. Instead, the increased susceptibility of these altered cells suggested that bactericidal action required permeation of a reagent through the cell envelope. In addition to altering the cell envelope, these procedures partly depleted cells of oxidizable substrates and sulfhydryl components. Adding an oxidizable substrate did not decrease the susceptibility of the altered cells. On the other hand, mild reducing agents such as sulfhydryl compounds did partly reverse bactericidal action when added after exposure of cells to the peroxidase systems. These studies indicate that alteration of the metabolism, structure, or composition of bacterial cells can greatly increase their susceptibility to peroxidase bactericidal action.
Authors:
E L Thomas; T M Aune
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  13     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1978 Feb 
Date Detail:
Created Date:  1978-06-12     Completed Date:  1978-06-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  261-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / drug effects*,  metabolism
Hydrogen Peroxide / metabolism
Iodides / pharmacology*
Lactoperoxidase / pharmacology*
Oxygen Consumption / drug effects
Peroxidases / metabolism,  pharmacology*
Peroxides / pharmacology*
Sulfhydryl Compounds / metabolism
Thiocyanates / pharmacology*
Chemical
Reg. No./Substance:
0/Iodides; 0/Peroxides; 0/Sulfhydryl Compounds; 0/Thiocyanates; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Lactoperoxidase; EC 1.11.1.-/Peroxidases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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