Document Detail


Survival and viability of vitrified in vitro and in vivo produced ovine blastocysts.
MedLine Citation:
PMID:  10883839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ovine blastocysts were produced by maturation, fertilization and in vitro culture (IVM/IVF/IVC) of oocytes from slaughtered adult and prepubertal ewes and collection from superovulated and inseminated adult animals. Dulbecco's PBS supplemented with 0.3 mM Na Pyruvate and 20% FCS was used as the basic cryopreservation solution. The embryos were exposed to the vitrification solution as follows: 10% glycerol (G) for 5 min, then 10% G +20% ethylene glycol (EG) for 5 min. Embryos were placed into 25% G + 25% EG in the center of 0.25- mL straws and plunged immediately into LN2. Warming was done by placing the straws into a water bath at 37 degrees C for 20 sec, and their contents were expelled into a 0.5 M sucrose solution for 3 min; the embryos were then transferred into 0.25 M and 0.125 M sucrose solution for 3 min each. Warmed blastocysts were transferred to the culture medium for 24 h. Survival was defined as the re-expansion of the blastocoele. All surviving blastocysts were transferred to synchronized recipient ewes, and the pregnancy was allowed to go to term. Of 68 vitrified in vitro produced blastocysts, 46 re-expanded (67.6%) and 10 lambs were born (14.7%). From the 62 in vivo derived and vitrified embryos, 52 re-expanded (83.8%) and 39 lambs were born (62.9%). The lambing rate of in vitro produced fresh transfer embryos was 40% (20 lambs/50 blastocysts transferred), and of the 32 in vivo derived blastocysts and transferred fresh, 26 lambs were born (81.2%). The results indicate that in vitro produced embryos can be successfully cryopreserved by vitrification.
Authors:
M Dattena; G Ptak; P Loi; P Cappai
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Theriogenology     Volume:  53     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-09-11     Completed Date:  2000-09-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1511-9     Citation Subset:  IM    
Affiliation:
Istituto Zootecnico e Caseario per la Sardegna, Servizio Riproduzione Animale, Sassari, Italy. mdattena@tiscalinet.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / physiology*
Cryopreservation / methods,  veterinary*
Embryo Transfer / veterinary
Estrus Synchronization / physiology
Female
Fertilization in Vitro / methods,  veterinary*
Male
Ovarian Follicle / physiology
Pregnancy
Pregnancy Outcome / veterinary
Sheep / embryology,  physiology*
Superovulation / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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