Document Detail

Survival of unprotected, mammalian plateau-phase cells following freezing in liquid nitrogen.
MedLine Citation:
PMID:  971589     Owner:  NLM     Status:  MEDLINE    
Unprotected, mammalian cells in plateau phase are at least a factor of four times more sensitive to freeze-thaw damage than exponential-phase cells. The former suffer about 15-20% more sublethal damage after one freeze-thaw cycle than the latter and repair this damage more slowly. Exposure of plateau-phase cells to freeze-thaw damage lengthens the time required to traverse the cell cycle in the exposed generation. These cells may more closely represent the state in tissues than exponential-phase populations.
J Frim; J Kruuv; H E Frey; G P Raaphorst
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cryobiology     Volume:  13     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1976 Aug 
Date Detail:
Created Date:  1976-12-03     Completed Date:  1976-12-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  475-83     Citation Subset:  IM    
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MeSH Terms
Cell Survival*
Nitrogen / pharmacology*
Tissue Preservation*
Reg. No./Substance:

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