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Survival of silage lactic acid bacteria in the goat gastrointestinal tract as determined by denaturing gradient gel electrophoresis.
MedLine Citation:
PMID:  22925065     Owner:  NLM     Status:  Publisher    
Aims:  To determine the survival rate of silage lactic acid bacteria (LAB) in the ruminant gastrointestinal tract. Methods and Results:  Wilted Italian ryegrass (Lolium multiflorum Lam.) silage (containing 1.9 × 10(6) cfu LAB·g(-1) ) was fed ad libitum to 3 goats equipped with rumen cannulae. Silage was given alone or with concentrates at a 1:1 ratio on a dry matter basis. Rumen fluid was then obtained 2, 4, and 8 h after the morning feeding. Denaturing gradient gel electrophoresis was performed to compare LAB communities in silage, rumen fluid, and faeces. The LAB detected in the wilted silage included Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus murinus, and Lactobacillus sakei. Bands indicative of L. murinus were detected in either the rumen fluid or faeces whereas the bands indicative of L. plantarum, L. brevis, and L. sakei were not. Although the rumen fluid LAB counts and volatile fatty acid concentrations were higher in goats fed silage plus concentrates compared with those fed silage alone, the LAB communities themselves remained unaffected. Sampling times and goat-to-goat variations did not affect the LAB communities found in the rumen fluid. Conclusion:  LAB communities found in the gut are not remarkably affected by the consumption of silage LAB, even when the silage is accompanied by concentrates that facilitate gut fermentation. © 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.
Hongyan Han; Shota Takase; Naoki Nishino
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-25
Journal Detail:
Title:  Letters in applied microbiology     Volume:  -     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.
Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
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