| Survival and phase sensitivity of HeLa cells treated with dianhydrogalactitol (NSC-132313). | |
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MedLine Citation:
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PMID: 1203879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of dianhydrogalactitol on survival and phase sensitivity of the cell cycle of HeLa cells was studied. In survival experiments no cells survived when the drug was added and left in contact with the cells at or above a dose of 0.7 mug/ml. Cells synchronized by the mitotic-selection method showed changing sensitivity to the drug during the cell cycle. G, late-S, and M phases were more sensitive to dianhydrogalactitol than the early-S and G phases. The DNA synthetic phase was divided into an early low sensitive and a late highly sensitive stage. The different sensitivity of mid-G and early-S phases was further established in separate survival experiments. Cells treated in G phase entered M phase in a manner similar to untreated control cells, while the progress of cells in S or G phase during treatment was delayed significantly. Lack of phase specificity of this drug is consistent with that of other alkylating agents. Changing sensitivity during some phases of the cell cycle proves that some metabolic events are especially sensitive to this particular drug. |
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Authors:
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I Pályi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cancer chemotherapy reports. Part 1 Volume: 59 ISSN: 0069-0112 ISO Abbreviation: Cancer Chemother Rep Publication Date: 1975 May-Jun |
Date Detail:
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Created Date: 1976-03-30 Completed Date: 1976-03-30 Revised Date: 2008-02-20 |
Medline Journal Info:
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Nlm Unique ID: 7607105 Medline TA: Cancer Chemother Rep Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 493-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division
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drug effects Cell Survival / drug effects DNA Replication / drug effects Dose-Response Relationship, Drug Ethers, Cyclic / pharmacology Hela Cells / drug effects* Mitosis / drug effects Sugar Alcohols / pharmacology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Ethers, Cyclic; 0/Sugar Alcohols |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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