Document Detail

Survival, migration, and differentiation of Sox1-GFP embryonic stem cells in coculture with an auditory brainstem slice preparation.
MedLine Citation:
PMID:  18241123     Owner:  NLM     Status:  MEDLINE    
The poor regeneration capability of the mammalian hearing organ has initiated different approaches to enhance its functionality after injury. To evaluate a potential neuronal repair paradigm in the inner ear and cochlear nerve we have previously used embryonic neuronal tissue and stem cells for implantation in vivo and in vitro. At present, we have used in vitro techniques to study the survival and differentiation of Sox1-green fluorescent protein (GFP) mouse embryonic stem (ES) cells as a monoculture or as a coculture with rat auditory brainstem slices. For the coculture, 300 microm-thick brainstem slices encompassing the cochlear nucleus and cochlear nerve were prepared from postnatal SD rats. The slices were propagated using the membrane interface method and the cochlear nuclei were prelabeled with DiI. After some days in culture a suspension of Sox1 cells was deposited next to the brainstem slice. Following deposition Sox1 cells migrated toward the brainstem and onto the cochlear nucleus. GFP was not detectable in undifferentiated ES cells but became evident during neural differentiation. Up to 2 weeks after transplantation the cocultures were fixed. The undifferentiated cells were evaluated with antibodies against progenitor cells whereas the differentiated cells were determined with neuronal and glial markers. The morphological and immunohistochemical data indicated that Sox1 cells in monoculture differentiated into a higher percentage of glial cells than neurons. However, when a coculture was used a significantly lower percentage of Sox1 cells differentiated into glial cells. The results demonstrate that a coculture of Sox1 cells and auditory brainstem present a useful model to study stem cell differentiation.
Aleksandra Glavaski-Joksimovic; Charoensri Thonabulsombat; Malin Wendt; Mikael Eriksson; Björn Palmgren; Anna Jonsson; Petri Olivius
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cloning and stem cells     Volume:  10     ISSN:  1536-2302     ISO Abbreviation:  Cloning Stem Cells     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-04     Completed Date:  2008-06-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101125444     Medline TA:  Cloning Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  75-88     Citation Subset:  IM    
Center for Hearing and Communication Research, Karolinska University Hospital, Stockholm, Sweden.
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MeSH Terms
Animals, Newborn
Auditory Cortex / physiology*
Brain Stem / physiology*
Cell Differentiation / physiology*
Cell Movement / physiology*
Cell Survival
Coculture Techniques
DNA-Binding Proteins / metabolism*
Embryo, Mammalian
Embryonic Stem Cells / metabolism,  physiology*
Green Fluorescent Proteins / metabolism*
High Mobility Group Proteins / metabolism*
Mice, Inbred C57BL
Mice, Transgenic
Rats, Sprague-Dawley
Recombinant Fusion Proteins / metabolism
SOXB1 Transcription Factors
Reg. No./Substance:
0/DNA-Binding Proteins; 0/High Mobility Group Proteins; 0/Recombinant Fusion Proteins; 0/SOXB1 Transcription Factors; 0/Sox1 protein, mouse; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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