Document Detail


SURVIVAL OF LACTOBACILLUS LEICHMANNII IN RELATION TO VITAMIN B12 ASSAYS.
MedLine Citation:
PMID:  14325293     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since washed cells of Lactobacillus leichmannii ATCC 7830 frequently showed erratic results in vitamin B(12) assays, a depletion technique was used to stabilize the inoculum. The method consisted of incubating the twice-washed cells at 37 C for 45 min in distilled water. Survival and growth studies indicated that the stabilizing procedure (i) did not affect cell numbers, (ii) was optimal for depleting possible carry-over of vitamin B(12) or other nutrilites and reserves, and (iii) brought about a phasing of the cells of the inoculum which underwent logarithmic growth immediately on inoculation. This minimal period (45 min) was the same when the cells were incubated in dextrose-water. Survival of the cells in physiological saline was greater than in distilled water, but a longer time was required for stabilizing the inoculum in saline, which precluded its use in routine work. The prepared inoculum showed an improvement over the washed inoculum in that readings between duplicate and triplicate tubes agreed closely and the standard curves were reproducible whether acidimetric or turbidimetric methods were followed. No erratic results were noted in more than 1 year of testing.
Authors:
J A VALU
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  13     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1965 May 
Date Detail:
Created Date:  1965-11-01     Completed Date:  1996-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  486-90     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Culture Media*
Lactobacillus*
Metabolism*
Research*
Vitamin B 12*
Chemical
Reg. No./Substance:
0/Culture Media; 68-19-9/Vitamin B 12
Comments/Corrections

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