| Survival in subpopulations of cells derived from solid KHT sarcomas by centrifugal elutriation following treatment with CCNU and MISO. | |
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MedLine Citation:
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PMID: 6480449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Misonidazole (MISO) has been shown to enhance the cytotoxicity of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) in a number of different animal tumor systems. We have investigated the response to therapy of the various subpopulations of cells comprising the KHT sarcoma, to determine whether chemopotentiation occurred as a preferential enhancement of killing in one subpopulation of cells. Twenty-four hr after drug treatment, cells dissociated from solid tumors were separated into homogeneous populations based on cell size by the technique of centrifugal elutriation. By this method the majority of the non-neoplastic cells could be removed and the tumor cells separated into fractions containing 90 to 95% G1 cells, 70 to 75% S cells and 70 to 80% G2M cells. Clonogenic cell survival was measured for each elutriated fraction. In vivo treatment with 0.5 mg/g MISO produced no measurable cell-kill across the cell cycle. Those cells in late G1 and S phase 24 hr after treatment were most sensitive to CCNU alone. The enhancement of CCNU cytotoxicity by MISO occurred primarily in the early G1 and S fractions. These data suggest that chemopotentiation does not occur equally in all tumor cell subpopulations and that some specificity of enhanced cell killing exists. |
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Authors:
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S A Hill; P C Keng; D W Siemann |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: International journal of radiation oncology, biology, physics Volume: 10 ISSN: 0360-3016 ISO Abbreviation: Int. J. Radiat. Oncol. Biol. Phys. Publication Date: 1984 Sep |
Date Detail:
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Created Date: 1984-11-16 Completed Date: 1984-11-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7603616 Medline TA: Int J Radiat Oncol Biol Phys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1615-8 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Cell Separation Cell Survival / drug effects Drug Synergism Drug Therapy, Combination Flow Cytometry Lomustine / therapeutic use* Mice Mice, Inbred C3H Misonidazole / therapeutic use* Neoplasm Transplantation Nitroimidazoles / therapeutic use* Radiation-Sensitizing Agents / therapeutic use Sarcoma, Experimental / drug therapy* |
| Grant Support | |
ID/Acronym/Agency:
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CA-11051/CA/NCI NIH HHS; CA-11198/CA/NCI NIH HHS; CA-20329/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nitroimidazoles; 0/Radiation-Sensitizing Agents; 13010-47-4/Lomustine; 13551-87-6/Misonidazole |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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