Document Detail

Survival of honey bee (Hymenoptera: Apidae) spermatozoa incubated at room temperature from drones exposed to miticides.
MedLine Citation:
PMID:  18767713     Owner:  NLM     Status:  MEDLINE    
We conducted research to examine the potential impacts ofcoumaphos, fluvalinate, and Apilife VAR (Thymol) on drone honey bee, Apis mellifera L. (Hymenoptera: Apidae), sperm viability over time. Drones were reared in colonies that had been treated with each miticide by using the dose recommended on the label. Drones from each miticide treatment were collected, and semen samples were pooled. The pooled samples from each treatment were subdivided and analyzed for periods of up to 6 wk. Random samples were taken from each treatment (n = 6 pools) over the 6-wk period. Sperm viability was measured using dual-fluorescent staining techniques. The exposure of drones to coumaphos during development and sexual maturation significantly reduced sperm viability for all 6 wk. Sperm viability significantly decreased from the initial sample to week 1 in control colonies, and a significant decrease in sperm viability was observed from week 5 to week 6 in all treatments and control. The potential impacts of these results on queen performance and failure are discussed.
Lisa M Burley; Richard D Fell; Richard G Saacke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of economic entomology     Volume:  101     ISSN:  0022-0493     ISO Abbreviation:  J. Econ. Entomol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-09-04     Completed Date:  2008-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985127R     Medline TA:  J Econ Entomol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1081-7     Citation Subset:  IM    
Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 42061, USA.
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MeSH Terms
Bees / drug effects*,  parasitology
Insecticides / adverse effects*
Semen Preservation
Spermatozoa / drug effects*
Reg. No./Substance:

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