Document Detail

Survival and germination of Bacillus cereus spores without outgrowth or enterotoxin production during in vitro simulation of gastrointestinal transit.
MedLine Citation:
PMID:  22923409     Owner:  NLM     Status:  MEDLINE    
To study the gastrointestinal survival and enterotoxin production of the food-borne pathogen Bacillus cereus, an in vitro simulation experiment was developed to mimic gastrointestinal passage in 5 phases: (i) the mouth, (ii) the stomach, with gradual pH decrease and fractional emptying, (iii) the duodenum, with high concentrations of bile and digestive enzymes, (iv) dialysis to ensure bile reabsorption, and (v) the ileum, with competing human intestinal bacteria. Four different B. cereus strains were cultivated and sporulated in mashed potato medium to obtain an inoculum of 7.0 log spores/ml. The spores showed survival and germination during the in vitro simulation of gastrointestinal passage, but vegetative outgrowth of the spores was suppressed by the intestinal bacteria during the final ileum phase. No bacterial proliferation or enterotoxin production was observed, despite the high inoculum levels. Little strain variability was observed: except for the psychrotrophic food isolate, the spores of all strains survived well throughout the gastrointestinal passage. The in vitro simulation experiments investigated the survival and enterotoxin production of B. cereus in the gastrointestinal lumen. The results obtained support the hypothesis that localized interaction of B. cereus with the host's epithelium is required for diarrheal food poisoning.
Siele Ceuppens; Mieke Uyttendaele; Katrien Drieskens; Marc Heyndrickx; Andreja Rajkovic; Nico Boon; Tom Van de Wiele
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-24
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-08     Completed Date:  2013-04-08     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7698-705     Citation Subset:  IM    
Ghent University, Faculty of Bioscience Engineering, Laboratory of Food Microbiology and Food Preservation (LFMFP), Ghent, Belgium.
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MeSH Terms
Bacillus cereus / physiology*
Colony Count, Microbial
Culture Media
Duodenum / microbiology
Enterotoxins / biosynthesis*
Feces / microbiology
Foodborne Diseases / microbiology
Gastrointestinal Tract / microbiology*
Gastrointestinal Transit
Intestinal Mucosa / microbiology*
Solanum tuberosum / microbiology
Spores, Bacterial / physiology*
Reg. No./Substance:
0/Culture Media; 0/Enterotoxins; 0/enterotoxin, Bacillus cereus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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