Document Detail


Survival and germination of Bacillus cereus spores without outgrowth or enterotoxin production during in vitro simulation of gastrointestinal transit.
MedLine Citation:
PMID:  22923409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study the gastrointestinal survival and enterotoxin production of the food-borne pathogen Bacillus cereus, an in vitro simulation experiment was developed to mimic gastrointestinal passage in 5 phases: (i) the mouth, (ii) the stomach, with gradual pH decrease and fractional emptying, (iii) the duodenum, with high concentrations of bile and digestive enzymes, (iv) dialysis to ensure bile reabsorption, and (v) the ileum, with competing human intestinal bacteria. Four different B. cereus strains were cultivated and sporulated in mashed potato medium to obtain an inoculum of 7.0 log spores/ml. The spores showed survival and germination during the in vitro simulation of gastrointestinal passage, but vegetative outgrowth of the spores was suppressed by the intestinal bacteria during the final ileum phase. No bacterial proliferation or enterotoxin production was observed, despite the high inoculum levels. Little strain variability was observed: except for the psychrotrophic food isolate, the spores of all strains survived well throughout the gastrointestinal passage. The in vitro simulation experiments investigated the survival and enterotoxin production of B. cereus in the gastrointestinal lumen. The results obtained support the hypothesis that localized interaction of B. cereus with the host's epithelium is required for diarrheal food poisoning.
Authors:
Siele Ceuppens; Mieke Uyttendaele; Katrien Drieskens; Marc Heyndrickx; Andreja Rajkovic; Nico Boon; Tom Van de Wiele
Related Documents :
20382099 - Epidemiology of cyclospora cayetanensis: a review focusing in endemic areas.
3183409 - An outbreak of food-borne campylobacter jejuni infection and the possible role of cross...
188789 - Laboratory confirmation of an outbreak of clostridium perfringens food poisoning.
19134229 - Outbreaks of shigella sonnei infections in denmark and australia linked to consumption ...
10575769 - A case-control study of yersinia enterocolitica infections in auckland.
937629 - An outbreak of giardiasis in a group of campers.
1698509 - Peptide-amine interactions in the hypothalamic paraventricular nucleus: analysis of gal...
11244249 - Food-related gastrointestinal symptoms in the irritable bowel syndrome.
24884099 - Assimilation of polybrominated diphenyl ethers from microplastics by the marine amphipo...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-24
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-08     Completed Date:  2013-04-08     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7698-705     Citation Subset:  IM    
Affiliation:
Ghent University, Faculty of Bioscience Engineering, Laboratory of Food Microbiology and Food Preservation (LFMFP), Ghent, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacillus cereus / physiology*
Colony Count, Microbial
Culture Media
Duodenum / microbiology
Enterotoxins / biosynthesis*
Feces / microbiology
Foodborne Diseases / microbiology
Gastrointestinal Tract / microbiology*
Gastrointestinal Transit
Intestinal Mucosa / microbiology*
Solanum tuberosum / microbiology
Spores, Bacterial / physiology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Enterotoxins; 0/enterotoxin, Bacillus cereus
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular characterization of fluoroquinolone-resistant Aeromonas spp. isolated from imported shrimp...
Next Document:  Detection of botulinum neurotoxin serotype A, B, and F proteolytic activity in complex matrices with...