| Survival and germination of Bacillus cereus spores without outgrowth or enterotoxin production during in vitro simulation of gastrointestinal transit. | |
MedLine Citation:
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PMID: 22923409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To study the gastrointestinal survival and enterotoxin production of the food-borne pathogen Bacillus cereus, an in vitro simulation experiment was developed to mimic gastrointestinal passage in 5 phases: (i) the mouth, (ii) the stomach, with gradual pH decrease and fractional emptying, (iii) the duodenum, with high concentrations of bile and digestive enzymes, (iv) dialysis to ensure bile reabsorption, and (v) the ileum, with competing human intestinal bacteria. Four different B. cereus strains were cultivated and sporulated in mashed potato medium to obtain an inoculum of 7.0 log spores/ml. The spores showed survival and germination during the in vitro simulation of gastrointestinal passage, but vegetative outgrowth of the spores was suppressed by the intestinal bacteria during the final ileum phase. No bacterial proliferation or enterotoxin production was observed, despite the high inoculum levels. Little strain variability was observed: except for the psychrotrophic food isolate, the spores of all strains survived well throughout the gastrointestinal passage. The in vitro simulation experiments investigated the survival and enterotoxin production of B. cereus in the gastrointestinal lumen. The results obtained support the hypothesis that localized interaction of B. cereus with the host's epithelium is required for diarrheal food poisoning. |
Authors:
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Siele Ceuppens; Mieke Uyttendaele; Katrien Drieskens; Marc Heyndrickx; Andreja Rajkovic; Nico Boon; Tom Van de Wiele |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-08-24 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 78 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-08 Completed Date: 2013-04-08 Revised Date: 2013-07-12 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 7698-705 Citation Subset: IM |
Affiliation:
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Ghent University, Faculty of Bioscience Engineering, Laboratory of Food Microbiology and Food Preservation (LFMFP), Ghent, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus cereus
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physiology* Colony Count, Microbial Culture Media Duodenum / microbiology Enterotoxins / biosynthesis* Feces / microbiology Foodborne Diseases / microbiology Gastrointestinal Tract / microbiology* Gastrointestinal Transit Intestinal Mucosa / microbiology* Solanum tuberosum / microbiology Spores, Bacterial / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Enterotoxins; 0/enterotoxin, Bacillus cereus |
| Comments/Corrections | |
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