Document Detail

Survival and function of isolated hepatocytes after cryopreservation.
MedLine Citation:
PMID:  10418967     Owner:  NLM     Status:  MEDLINE    
Cryopreservation in liquid nitrogen is presently the only way for long-term storage of isolated hepatocytes. Freeze-thaw conditions are not well defined yet; the most critical parameters appear to be the choice of the cryoprotectant, composition of the freezing medium, and cooling and thawing rates. Comparable results have been obtained with hepatocytes from various species, including man. Cryopreservation usually results in low cell recovery and early alterations of functional activities. However, both phase I and phase II xenobiotic metabolism is still active after thawing, at least during a short period. Moreover, survival and function of cryopreserved hepatocytes can be improved when these cells have a high energy status, are cryopreserved after immobilization in a gel, separated from dead cells on a Percoll gradient or placed in more favorable culture conditions (e.g. in coculture with liver non parenchymal cells). Additional studies are needed to improve freeze-thaw protocols and to better characterize liver parenchymal cells after storage, including evaluation of their responsiveness to specific inducers.
A Guillouzo; L Rialland; A Fautrel; C Guyomard
Related Documents :
7033147 - Glycosphingolipids of k562 cells: a chemical and immunological analysis.
5000867 - Repair of injury in freeze-dried salmonella anatum.
3427217 - Metabolic integrity and stability of isolated rat brown adipocytes.
16758617 - Evaluation of mutagenic activity of dioxazid by the polyorgan micronuclear method in ex...
10824677 - Spontaneous activity in developing turtle retinal ganglion cells: statistical analysis.
18547617 - Insights from a whole cystectomy specimen--association of primary small cell carcinoma ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Chemico-biological interactions     Volume:  121     ISSN:  0009-2797     ISO Abbreviation:  Chem. Biol. Interact.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-08-04     Completed Date:  1999-08-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0227276     Medline TA:  Chem Biol Interact     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  7-16     Citation Subset:  IM    
INSERM U 456, Faculté de Pharmacie, Université de Rennes, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Survival / physiology
Cryopreservation / methods*
Liver* / cytology,  physiology
Organ Preservation / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Overview: hepatocytes and cryopreservation--a personal historical perspective.
Next Document:  Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applica...